11/08/2020
CM PRODUCTS PASSED ASTM E3135-18 STANDARD TEST
DETERMINING ANTIMICROBIAL EFFICACY OF ULTRAVIOLET GERMICIDAL IRRADIATION AGAINST MICROORGANISM ON CARRIERS WITH SIMULATED SOIL PER ASTM E3135-18
Green Cyber Inc. supplied an ultraviolet (UVC) disinfection device (Figure 1) for testing against ASTM E3135-18 “Standard Practice for Determining Antimicrobial Efficacy of Ultraviolet Germicidal Irradiation Against Microorganisms on Carriers with Simulated Soil” test method at GAP EnviroMicrobial Services Ltd. (GAP).
The carriers used in this test consisted of painted glass slides and were placed in three independent locations on the payment terminal/debit machine (Figure 2). Prior to testing, each carrier was sterilized using a chemical disinfectant and dried so that no residual remained. The carriers placed at each location were separately inoculated with 10 x 1µL droplets of the challenge microbe, after the droplets dried (~15 minutes) each spot was overlaid with a 1µL droplet of fetal bovine serum (soiling agent) and allowed to dry (~15 minutes). Carriers were then placed in the device, as shown in Figure 3. After the exposure time, the surviving microbes were eluted by placing the carriers in a 500mL bottle containing 25mL of DE neutralization buffer and agitated for 1 minute. Control carriers were treated similarly, except no UV cycle was exposed prior to eluting the surviving microbes. The surviving microbes were enumerated using a pour plate method. Log and percent reductions were calculated by comparison to control carriers.
