PAKAR Laboratory

02/09/2025

17/08/2025

11/06/2025

Molecular Super Intelligence in the age of Digital Biology🎯✅

💥1️⃣ Imagine a source of new molecules with unprecedented chemical and structural diversity.

📌Medicinal fungal fermentation
technology platform | MFFTP.

💥2️⃣Imagine a system of multi-tier chemical separation so powerful that it can generate over 132,000,000 fractions at industrial scale.

📌Ultra-deep chemical analysis and
separation technology platform
| UDCASTP.

💥3️⃣Imagine large libraries of new chemical entities of more than 1,000,000,000, capable of producing hits against any biological target, any biological mechanism and any biological question.

📌 Libraries of active
pharmaceutical ingredients with
unprecedented chemical
structural diversity and
mechanisms of action | APILTP.

💥4️⃣Imagine a wide array bioassays to interrogate huge numbers of biological targets and biological mechanisms more than 1,000,000 across all life forms.

📌Chemical Systems Biology using
multi-omics technologies
| CSB-MOT

🎁 Finally imagine employing these powerful machines to produce data of unprecedented depths and scope unveiling master regulators and master mechanisms leading to most definitive and decisive solutions to global health and one health challenges.

⛔Challenges such as cancer, neglected tropical diseases, antimicrobial resistance, emergence of new hypervigilant pathogens, pandemics and aging.

🆔️PAKARLab and 🆔️PAKARLabs our vision and mission is audacious but we will proceed inspite of formidable odds.

04/06/2025

A great company to work with.

03/06/2025

Some of these fluorescent compounds come alive even in the very dilute fraction.

The roads to significant discoveries and major breakthroughs are paved with dedication to a strong work ethic.

26/03/2025

Celebrating my 13th year on Facebook. Thank you for your continuing support. I could never have made it without you. 🙏🤗🎉

23/11/2023

AMR alert

12/10/2023

Codeine dysregulates ribosome biogenesis in Escherichia coli with DNA double-strand breaks to chart path to new classes of antibiotics

https://www.future-science.com/doi/10.4155/fdd-2023-0005

Abstract
Aim: A bacterial genetics-guided approach was utilized for the discovery of new compounds affecting bacterial genome stability. Materials & methods: Fungal extracts and fractions were tested for genome instability-mediated antibacterial activity. Interaction assays and RT-qPCR were used to identify compounds that boost the activity of sub-minimum inhibitory concentration streptomycin and obtain insights on the molecular mechanisms of the primary hit compound, respectively. Results: Several extracts and fractions caused bacterial genome instability. Codeine, in synergy with streptomycin, regulates double-strand break (DSB) repair and causes bacterial ribosome dysfunction in the absence of DSBs, and dysregulation of ribosome biogenesis in a DSB-dependent manner. Conclusion: This study demonstrates a potential viable strategy that we are exploring for the discovery of new chemical entities with activities against Escherichia coli and other bacterial pathogens.

30/03/2023

Simple, durable and versatile tool kit for multiple applications in the laboratory.

22/09/2022

Mission in motion

With our own stack of BIORECTORS

15/06/2022

Imagination is more important than knowledge: Inventing a TLC sprayer

We live by this idea daily in our small and struggling laboratory for chemical systems biology. Struggling yet bold with our ideas and ambitions.

This is a story of the power improvisation within low resources settings. For months now we have been trying to procure spraying device for thin layer chromatography. The device we wanted is a bit expensive and the procurement is taking forever.

So got to work to find a local replacement
which will work fine. All the options we tried fall apart after a few rounds of use due to the strong acid in the staining reagent.

So we went back to town and found this item that looks like it's made with durable plastic. It's used for spreading insecticides and cleaning solutions.

We could vouch for the spraying mechanism to survive so this quick thinking brother decided to separate the problem into two and limit the exposure of strong acid only to the nozzle side and spare the air pump.

He tried a long test tube first and then we thought of this little flask. At least it reduces the number of times we need to refill.

We tested it and worked beautifully. The nozzle is tunable which allows us to obtain super fine spraying. And it is very durable too. We have used it intensely for two months now and it's going strong.

Again our quick thinking brother decided to be over cautious. And he rinses the device with deionized water each time after use in order not to give the strong acid the chance to drift into the air pump side. The soft plastics that allows the piston to deliver the pressure will not survive.

It looks like with this strategy we can going to have this improvised device around for a long time. And costed us just about 7 USD instead 190 USD that would have taken forever to arrive.

Feel free and set one for your Laboratory if you don't have a good sprayer at hand. This is better than pouring the reagent over the plate or even dipping into a tank of reagent.