Medicuate

Medicuate Welcome to Medicuate, your ultimate medical hub! We guide students and empower all with vital health

30/07/2025

๐Ÿ”ฌ Calibration vs Validation โ€“ Know the Difference!

In the world of pharmaceuticals and analytical chemistry, precision and compliance are non-negotiable. But many still confuse two essential terms: Calibration and Validation.

Letโ€™s simplify it ๐Ÿ‘‡

๐Ÿ“ Calibration
โžก๏ธ Ensures instruments are giving accurate, traceable results.
โžก๏ธ Example: Calibrating a balance with standard weights to ensure proper readings.
โœ… Itโ€™s all about adjusting the instrument.

๐Ÿงช Validation
โžก๏ธ Proves a method, process, or system consistently produces reliable results.
โžก๏ธ Example: Validating an HPLC method for assay testing.
โœ… Itโ€™s about documented evidence that your method works as intended.

๐Ÿ’ก Both are regulatory requirements โ€” but they play very different roles in maintaining quality and compliance.

๐Ÿ‘ฉ๐Ÿ”ฌ Accuracy starts with calibration.
๐Ÿงพ Reliability is confirmed by validation.

๐˜œ๐˜ฏ๐˜ญ๐˜ฐ๐˜ค๐˜ฌ๐˜ช๐˜ฏ๐˜จ ๐˜‹๐˜•๐˜ˆ ๐˜š๐˜ฆ๐˜ค๐˜ณ๐˜ฆ๐˜ต๐˜ด: ๐˜›๐˜ฉ๐˜ฆ ๐˜Œ๐˜ด๐˜ด๐˜ฆ๐˜ฏ๐˜ต๐˜ช๐˜ข๐˜ญ ๐˜™๐˜ฆ๐˜ข๐˜จ๐˜ฆ๐˜ฏ๐˜ต๐˜ด ๐˜‰๐˜ฆ๐˜ฉ๐˜ช๐˜ฏ๐˜ฅ ๐˜—๐˜Š๐˜™! โฃโฃโฃโฃPolymerase Chain Reaction (PCR) has revolutionized molecul...
27/07/2025

๐˜œ๐˜ฏ๐˜ญ๐˜ฐ๐˜ค๐˜ฌ๐˜ช๐˜ฏ๐˜จ ๐˜‹๐˜•๐˜ˆ ๐˜š๐˜ฆ๐˜ค๐˜ณ๐˜ฆ๐˜ต๐˜ด: ๐˜›๐˜ฉ๐˜ฆ ๐˜Œ๐˜ด๐˜ด๐˜ฆ๐˜ฏ๐˜ต๐˜ช๐˜ข๐˜ญ ๐˜™๐˜ฆ๐˜ข๐˜จ๐˜ฆ๐˜ฏ๐˜ต๐˜ด ๐˜‰๐˜ฆ๐˜ฉ๐˜ช๐˜ฏ๐˜ฅ ๐˜—๐˜Š๐˜™! โฃโฃ
โฃโฃ
Polymerase Chain Reaction (PCR) has revolutionized molecular biology, but what actually fuels this powerful technique?โฃโฃ
The key reagents that make DNA amplification possible are:โฃโฃ
โฃโฃ
๐Ÿ. ๐ƒ๐๐€ ๐“๐ž๐ฆ๐ฉ๐ฅ๐š๐ญ๐žโฃโฃ
The sample DNA that contains the target region to be amplified.โฃโฃ
โฃโฃ
๐Ÿ. ๐๐ซ๐ข๐ฆ๐ž๐ซ๐ฌ (๐…๐จ๐ซ๐ฐ๐š๐ซ๐ & ๐‘๐ž๐ฏ๐ž๐ซ๐ฌ๐ž)โฃโฃ
Short, synthetic oligonucleotides that bind to the specific target sequence and initiate replication.โฃโฃ
โฃโฃ
๐Ÿ‘. ๐๐๐“๐๐ฌ (๐ƒ๐ž๐จ๐ฑ๐ฒ๐ง๐ฎ๐œ๐ฅ๐ž๐จ๐ญ๐ข๐๐ž ๐“๐ซ๐ข๐ฉ๐ก๐จ๐ฌ๐ฉ๐ก๐š๐ญ๐ž๐ฌ)โฃโฃ
Building blocks (A, T, G, C) for synthesizing new DNA strands.โฃโฃ
โฃโฃ
๐Ÿ’. ๐“๐š๐ช ๐ƒ๐๐€ ๐๐จ๐ฅ๐ฒ๐ฆ๐ž๐ซ๐š๐ฌ๐žโฃโฃ
A thermostable enzyme derived from Thermus aquaticus that extends primers and synthesizes DNA at high temperatures.โฃโฃ
โฃโฃ
๐Ÿ“. ๐๐ฎ๐Ÿ๐Ÿ๐ž๐ซ ๐’๐จ๐ฅ๐ฎ๐ญ๐ข๐จ๐งโฃโฃ
Maintains optimal pH and ionic conditions necessary for enzyme activity and stability.โฃโฃ
โฃโฃ
๐Ÿ”. ๐Œ๐ ๐‚๐ฅโ‚‚ (๐Œ๐š๐ ๐ง๐ž๐ฌ๐ข๐ฎ๐ฆ ๐‚๐ก๐ฅ๐จ๐ซ๐ข๐๐ž)โฃโฃ
A crucial cofactor that enhances the catalytic activity of DNA polymerase.โฃโฃ
โฃโฃ
All these components work synergistically in a thermo cycler to denature DNA, anneal primers, and extend new strands, completing cycles of exponential DNA amplification.โฃโฃ
โฃโฃ
Whether you're diagnosing disease, genotyping, cloning, or conducting forensic analysis, PCR wouldn't be possible without these fundamental reagents.โฃโฃ
โฃ โฃโฃ
โฃโฃโฃโฃโฃโ™ป๏ธ ๐˜™๐˜ฆ๐˜ฑ๐˜ฐ๐˜ด๐˜ต ๐˜ต๐˜ฉ๐˜ช๐˜ด ๐˜ช๐˜ง ๐˜บ๐˜ฐ๐˜ถ ๐˜ญ๐˜ฆ๐˜ข๐˜ณ๐˜ฏ๐˜ฆ๐˜ฅ ๐˜ด๐˜ฐ๐˜ฎ๐˜ฆ๐˜ต๐˜ฉ๐˜ช๐˜ฏ๐˜จ ๐˜ฏ๐˜ฆ๐˜ธ.โฃโฃโฃโฃโฃโฃโฃโฃโฃโฃโฃโฃโฃโฃโฃโฃโฃโฃโฃโฃ
โฃโฃโฃโฃโฃ

08/07/2025

Agarose Gel Electrophoresis for the Separation of DNA Fragments


Cred:JQVE

The First-year University of Mumbai medical ๐Ÿ‘จโ€โš•๏ธstudents were attending their 1st Anatomy Class. They all gathered aroun...
19/03/2025

The First-year University of Mumbai medical ๐Ÿ‘จโ€โš•๏ธstudents were attending their 1st Anatomy Class. They all gathered around the table. There was a real dead body on the table.

The Professor, Krishna, started the class by telling them two important qualities of a DOCTOR. He said, "The 1st is that never be DISGUSTED about anything in the body." For example, he inserted his FINGER in the dead body's A**S & put the finger in his mouth & TASTED it.

Then he told the students to do what he did. The students hesitated for several minutes, but eventually, everyone inserted their fingers in the body's A**S & tasted it. When everyone finished tasting their fingers, they were all Frowning...

Then the professor looked at them & said: "The most important 2nd Quality is "Observation".

I inserted my MIDDLE finger but tasted the 2nd finger... Now, learn to pay attention!!

"ALL the students fainted!!


20/02/2025

The river water at the is not fit for bathing, warns a report. How it can affect you?

# #

20/02/2025

The biggest of the country
The biggest mafia in the country, and hospitals, have become the center of looting the sick people, which has been exposed by Dainik Bhaskar, the biggest question is that this kind of looting cannot happen without the permission of the government?? Watch the video and decide who is responsible??

Source:x

14/02/2025

Whatโ€™s going on with todayโ€™s generation?๐Ÿคฆ

This type of show should be banned



*This video neither created or edited by me

Share itโค๏ธโ€”someone could benefit from this
21/01/2025

Share itโค๏ธโ€”someone could benefit from this

Share itโค๏ธโ€”someone could benefit from this
21/01/2025

Share itโค๏ธโ€”someone could benefit from this

Recommended Order for Drawing Evacuated Tubes & Filling Tubes From a Syringe #
26/12/2024

Recommended Order for Drawing Evacuated Tubes & Filling Tubes From a Syringe

#

Address

Delhi

Website

Alerts

Be the first to know and let us send you an email when Medicuate posts news and promotions. Your email address will not be used for any other purpose, and you can unsubscribe at any time.

Contact The Practice

Send a message to Medicuate:

Shortcuts

Share

Share on Facebook Share on Twitter Share on LinkedIn
Share on Pinterest Share on Reddit Share via Email
Share on WhatsApp Share on Instagram Share on Telegram

Category