27/07/2025
𝘜𝘯𝘭𝘰𝘤𝘬𝘪𝘯𝘨 𝘋𝘕𝘈 𝘚𝘦𝘤𝘳𝘦𝘵𝘴: 𝘛𝘩𝘦 𝘌𝘴𝘴𝘦𝘯𝘵𝘪𝘢𝘭 𝘙𝘦𝘢𝘨𝘦𝘯𝘵𝘴 𝘉𝘦𝘩𝘪𝘯𝘥 𝘗𝘊𝘙!
Polymerase Chain Reaction (PCR) has revolutionized molecular biology, but what actually fuels this powerful technique?
The key reagents that make DNA amplification possible are:
𝟏. 𝐃𝐍𝐀 𝐓𝐞𝐦𝐩𝐥𝐚𝐭𝐞
The sample DNA that contains the target region to be amplified.
𝟐. 𝐏𝐫𝐢𝐦𝐞𝐫𝐬 (𝐅𝐨𝐫𝐰𝐚𝐫𝐝 & 𝐑𝐞𝐯𝐞𝐫𝐬𝐞)
Short, synthetic oligonucleotides that bind to the specific target sequence and initiate replication.
𝟑. 𝐝𝐍𝐓𝐏𝐬 (𝐃𝐞𝐨𝐱𝐲𝐧𝐮𝐜𝐥𝐞𝐨𝐭𝐢𝐝𝐞 𝐓𝐫𝐢𝐩𝐡𝐨𝐬𝐩𝐡𝐚𝐭𝐞𝐬)
Building blocks (A, T, G, C) for synthesizing new DNA strands.
𝟒. 𝐓𝐚𝐪 𝐃𝐍𝐀 𝐏𝐨𝐥𝐲𝐦𝐞𝐫𝐚𝐬𝐞
A thermostable enzyme derived from Thermus aquaticus that extends primers and synthesizes DNA at high temperatures.
𝟓. 𝐁𝐮𝐟𝐟𝐞𝐫 𝐒𝐨𝐥𝐮𝐭𝐢𝐨𝐧
Maintains optimal pH and ionic conditions necessary for enzyme activity and stability.
𝟔. 𝐌𝐠𝐂𝐥₂ (𝐌𝐚𝐠𝐧𝐞𝐬𝐢𝐮𝐦 𝐂𝐡𝐥𝐨𝐫𝐢𝐝𝐞)
A crucial cofactor that enhances the catalytic activity of DNA polymerase.
All these components work synergistically in a thermo cycler to denature DNA, anneal primers, and extend new strands, completing cycles of exponential DNA amplification.
Whether you're diagnosing disease, genotyping, cloning, or conducting forensic analysis, PCR wouldn't be possible without these fundamental reagents.
♻️ 𝘙𝘦𝘱𝘰𝘴𝘵 𝘵𝘩𝘪𝘴 𝘪𝘧 𝘺𝘰𝘶 𝘭𝘦𝘢𝘳𝘯𝘦𝘥 𝘴𝘰𝘮𝘦𝘵𝘩𝘪𝘯𝘨 𝘯𝘦𝘸.
