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شركة سينتفك آي للتجهيزات العلمية, مخيم قدورة/شارع المستشفى/عمارة الابراج 2, Ramallah (2026)

01/04/2022

Bile Esculin Agar Base
Intended Use:
Recommended for differential isolation and presumptive identification of group D Streptococci in food and pharmaceutical products.
Composition**
Ingredients Gms / Litre
Peptone 5.000
HM peptone B # 3.000
Bile 40.000
Ferric citrate 0.500
Agar 15.000
Final pH ( at 25°C) 6.6±0.2
**Formula adjusted, standardized to suit performance parameters # Equivalent to Meat extract
Principle And Interpretation
Group D Streptococci possess the group D lipoteichoic acid antigen in their cell walls. Former Group D species, which are predominant normal inhabitants of the human gastrointestinal tract, are termed as faecal Streptococci or Enterococci (7). The unique ability of Enterococci to split esculin was reported by Meyer and Schonfeld (9). Enterococci and Group D Streptococci hydrolyse esculin to esculetin and dextrose, which reacts with ferric citrate producing brownish black precipitate (8). The use of esculin hydrolysis in identification of Enterococci was first cited by Rochaix (11). Bile Esculin Agar was originally formulated by Swan (13) for the isolation and identification of Group D Streptococci from food. Facklam and Moody (2,3) further reported that using Bile Esculin Agar, Group D Streptococci could be differentiated from non Group D Streptococci. Bile Esculin Agar was also shown to aid differentiation of Enterobacteriaceae, Klebsiella, Enterobacter, Serratia from other Enterobacteriaceae genera (1) on the basis of esculin hydrolysis. However, other tests such as salt tolerance should be performed for identifying Enterococci (4).
Bile Esculin Agar Base with added supplements is recommended for selective isolation and presumptive identification of group D streptococci from food and pharmaceutical products.
Peptone and HM peptone B serves as sources of carbon, nitrogen, amino acids, vitamins and essential growth nutrients. Bile inhibits most of the other accompyning bacteria. Esculin when added as a supplement in the medium is hydrolyzed to esculetin and dextrose. Esculetin reacts with ferric citrate to form a dark brown or black complex, visualized as a zone of black precipitate around the colonies. If the media is dispensed in tubes in the form of slants, a positive reaction is indicated by blackening of more than half of the slant within 24-48 hours. If blackening is totally absent or if less than half of the slant is blackened within 24-48 hours, the test is negative. Viridans Streptococci sometimes exhibit a weak positive reaction.
Also, Leuconostoc, Pediococcus, Lactococcus species causing human infections give a positive bile esculin test (10). To enhance the growth of Enterococci, Bile Esculin Agar can be supplemented with 50ml/l horse serum (8). Inoculate and incubate the test sample in Todd Hewitt Broth (M313). After 24 hours incubation add two drops of the culture onto the surface of slant or plate media (4,8).
Type of specimen
Food samples; Pharmaceutical samples.
Specimen Collection and Handling
For food samples, follow appropriate techniques for sample collection and processing as per guidelines (12).
For pharmaceutical samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards.
After use, contaminated materials must be sterilized by autoclaving before discarding.

Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
1. Viridans Streptococci sometimes exhibit a weak positive reaction.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to brownish yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Amber coloured, clear to slightly opalescent solution with a bluish tinge forms in Petri plates or in tubes as slants.
Reaction
Reaction of 6.35% w/v aqueous solution at 25°C. pH : 6.6±0.2
pH
6.40-6.80
Cultural Response
Cultural characteristics observed with added Esculin (FD050) in an increased atmosphere of Carbon dioxide, after an incubation at 35-37°C for 18-24 hours .

Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Use before expiry date on the label.
Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).

01/04/2022

Chocolate Agar Base
Intended use
Recommended for the isolation of Neisseria gonorrhoeae from chronic and acute cases of gonococcal infections.

Composition**
Ingredients Gms / Litre
Proteose peptone 20.000
Dextrose (Glucose) 0.500
Sodium chloride 5.000
Disodium hydrogen phosphate 5.000
Agar 15.000
Final pH ( at 25°C) 7.3±0.2
**Formula adjusted, standardized to suit performance parameters

Principle And Interpretation
Neisseria gonorrhoeae is a gram-negative bacteria and the causative agent of gonorrhea, however it is also occasionally found in the throat. The cultivation medium for gonococci should ideally be a rich nutrients base with blood, either partially lysed or completely lysed. The diagnosis and control of gonorrhea have been greatly facilitated by improved laboratory
methods for detecting, isolating and studying N. gonorrhoeae.
Chocolate Agar Base, with the addition of supplements, gives excellent growth of the gonococcus without overgrowth by contaminating organisms. G.C. Agar (M434) can also be used in place of Chocolate Agar Base, which gives slightly better results than Chocolate Agar (4). The diagnosis and control of gonorrhea have been greatly facilitated by improved laboratory methods for detecting, isolating and studying N. gonorrhoea.
Interest in the cultural procedure for the diagnosis of gonococcal infection was stimulated by Ruys and Jens (9), Mcleod and co-workers (8), Thompson (7), Leahy and Carpenter (1), Carpenter, Leahy and Wilson (2) and Carpenter (10), who clearly demonstrated the superiority of this method over the microscopic technique. Chocolate Agar Base with addition of supplement not only supports the growth of the gonococcus in pure culture but also permits its development from the mixed flora encountered in chronic gonococcal infections. Carpenter (3) reported that this medium and Haemoglobin (FD022) is useful for cultural detection of the gonococcus.

Warning and Precautions :
In Vitro diagnostic Use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
1. Further biochemical tests must be carried out for confirmation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Basal medium: Light amber coloured clear to slightly opalescent gel. After addition of haemoglobin : Chocolate brown coloured opaque gel forms in Petri plates.
Reaction
Reaction of 4.5% w/v aqueous solution at 25°C. pH : 7.3±0.2
pH
7.10-7.50
Cultural Response
Cultural characteristics observed with added 2% haemoglobin solution (FD022),Yeast autolysate Supplement (FD027) or Vitamino Growth Supplement(FD025), after an incubation at 35-37°C for 40-48 hours.

Storage and Shelf Life
Store between 10-30°C in a tightly closed container and the prepared medium at 2-8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.

Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).

01/04/2022

Nutrient Agar Medium MM012
Nutrient agar medium is used as a general purpose culture medium which may be used as enriched medium by incorporating blood or other biological fluids in accordance with Indian Pharmacopoeia.
Composition**
Ingredients Gms / Litre
Peptone 10.000
Meat extract B # 10.000
Sodium chloride 5.000
Agar 12.000
pH after sterilization 7.3±0.1
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Beef extract
Principle And Interpretation
Nutrient Agar is a basic culture medium used for maintaining microorganisms (1), for purity checking prior to biochemical or serological testing. It is used for the cultivation and enumeration of bacteria, which are not particularly fastidious. In semisolid form it is used for maintenance of control or standard organisms. Indian Pharmacopoeia has recommended it for microbial limit tests of viable aerobic microorganism present in pharmaceutical substances (2).
Peptone and meat extract B provide the necessary nitrogen compounds, carbon, vitamins and also some trace ingredients. Sodium chloride maintains osmotic equilibrium. Nutrient media may be used as enriched media by the addition of 10% v/v blood or other biological fluids like ascitic fluid, serum etc.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.2% Agar gel
Colour and Clarity of prepared medium
Light yellow coloured clear to slightly opalescent gel forms in Petri plates
pH
7.20-7.40
Growth Promotion Test
Growth Promotion is carried out as per Indian Pharmacopoeia
Cultural Response
MM012: Cultural characteristics observed after an incubation at 35-37°C for 18-48 hours.
Storage and Shelf Life
Store below 30°C in tightly closed container and prepared medium at 2-8°C . Use before expiry date on the label.
Reference
1. Lapage S., Shelton J. and Mitchell T., 1970, 'Methods in Microbiology', Norris J. and Ribbons D. (ed.), Vol. 3A., Academic Press, London.
2. Indian Pharmacopoeia, 1996, Govt. of India, The Controller of Publications, Delhi.

Disclaimer :
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in this and other related HiMedia™ publications. The information contained in this publication is based on our research and development work and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.
HiMedia Laboratories Pvt. Ltd. A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email: techhelp@himedialabs.com

03/03/2022

SS Agar, Modified
Intended Use:
SS Agar (Salmonella Shigella Agar) Modified is used for the selective isolation and differentiation of Salmonella and
Shigella species from pathological specimens, suspected foodstuffs etc.
Composition**
Ingredients Gms / Litre
Peptone 5.000
HM Peptone B # 5.000
Lactose 10.000
Bile salts mixture 5.500
Sodium citrate 10.000
Sodium thiosulphate 8.500
Ferric citrate 1.000
Brilliant green 0.00033
Neutral red 0.025
Agar 12.000
Final pH ( at 25°C) 7.2±0.2
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Beef extract
Principle And Interpretation
Salmonella and Shigella are gram-negative, facultatively anaerobic, non-sporulating rods in the family Enterobacteriaceae . They are widely distributed in animals, infecting mainly the stomach and the intestinal tissues. SS Agar is recommended as differential and selective medium for the isolation of Salmonella and Shigella species from pathological specimens (5) and suspected foodstuffs (1,6,8,9) and for microbial limit test (7). SS Agar is a moderately selective medium in which gram- positive bacteria are inhibited by bile salts, brilliant green and sodium citrate.
Peptone and HM Peptone B provide essential growth nutrients. Lactose is the fermentable carbohydrate. Brilliant green, bile salts and thiosulphate selectively inhibit gram-positive and coliform organisms. Sodium thiosulphate is reduced by certain species of enteric organisms to sulphite and H2S gas. This reductive enzymatic process is attributed to thiosulphate reductase. Production of H2S gas is detected as an insoluble black precipitate of ferrous sulphide, formed upon reaction of H2S with ferric ions or ferric citrate, indicated by black centered colonies.
The high selectivity of Salmonella Shigella Agar allows the use of large inocula directly from faeces, re**al swabs or other materials suspected of containing pathogenic enteric bacilli. On fermentation of lactose by few lactose-fermenting normal intestinal flora, acid is produced which is indicated by change of colour from yellow to red by the pH indicator neutral red. Thus these organisms grow as red-pigmented colonies. Lactose non-fermenting organisms grow as translucent colourless colonies with or without black centers. Salmonella species exhibit colourless colonies with black centers resulting from H2S production. Shigella species form colourless colonies, which do not produce H2S. While using samples suspected of being exposed to treatments that might have damaged the viability of microorganisms due to processing of food materials or samples from patients under antibiotic treatment etc., previous enrichment in Selenite cystine Broth Base (M025) or Tetrathionate Broth Base (M032) is necessary. Inoculate SS Agar plates with the enriched culture. After incubation the suspicious colonies should be subcultured on differential media to be identified biochemically or serologically.
Type of specimen
Clinical: faeces, blood, re**al swabs; Suspected foodstuffs.
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (2,3). For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (1,6,8,9).
Warning and Precautions
In Vitro diagnostic use. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.

Limitations
1.The medium is highly selective and may be toxic to certain Salmonella or Shigella species. Hence it is recommended to use
to inoculate plates of less inhibitory media parallel to SS Agar, such as Hektoen Enteric Agar (M467) or Deoxycholate Citrate Agar (M065) for easier isolation of Shigella species (4).

Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Light yellow to pink homogeneous free flowing powder
Gelling
Firm, comparable with 1.2% Agar gel
Colour and Clarity of Prepared Medium
Reddish orange coloured clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 5.7% w/v aqueous solution at 25°C. pH : 7.2±0.2
pH 7.00-7.40
Storage and Shelf Life
Store between 10- 30°C in a tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. On opening, product should be properly stored dry, after tightly capping the bottle in order to prevent lump formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use. Product performance is best if used within stated expiry period.

Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (2,3).

03/03/2022

Divided Plates of 5% Blood agar with Eosin methylene Blue.

03/03/2022

Mannitol Salt Agar
Intended Use:
Recommended for the isolation of pathogenic Staphylococci from clinical and non-clinical samples.
Composition**

Ingredients Gms / Litre
Proteose peptone 10.000
HM peptone B # 1.000
Sodium chloride 75.000
D-Mannitol 10.000
Phenol red 0.025
Agar 15.000
Final pH ( at 25°C) 7.4±0.2
**Formula adjusted, standardized to suit performance parameters # - Equivalent to Beef extract
Principle And Interpretation
Staphylococci are widespread in nature, although they are mainly found on the skin, skin glands and mucous membranes of mammals and birds. The coagulase-positive species i.e Staphylococcus aureus is well documented as a human opportunistic pathogen. The ability to clot plasma continues to be the most widely used and accepted criterion
for the identification of pathogenic staphylococci associated with acute infections (10). Staphylococci have the unique
ability of growing on a high salt containing media (8). Isolation of coagulase-positive staphylococci on Phenol Red Mannitol Agar supplemented with 7.5%NaCl was studied by Chapman (2). The resulting Mannitol Salt Agar Base is recommended for the isolation of coagulase-positive staphylococci from cosmetics, milk, food and other specimens (10,
5,3,12,11). The additional property of lipase activity of Staphylococcus aureus can be detected by the addition of the Egg Yolk Emulsion (FD045). The lipase activity can be visualized as yellow opaque zones around the colonies (4). HM peptone B and proteose peptone supply essential growth factors and trace nutrients to the growing bacteria. Sodium chloride serves as an inhibitory agent against bacteria other than staphylococci. Mannitol is the fermentable carbohydrate, fermentation of which leads to acid production, detected by phenol red indicator.
S.aureus ferment mannitol and produce yellow coloured colonies surrounded by yellow zones. Coagulase-negative strains of S.aureus are usually mannitol non-fermenters and therefore produce pink to red colonies surrounded by red-purple zones. Presumptive coagulase-positive yellow colonies of S. aureus should be confirmed by performing the coagulase test [tube or slide] (10). Lipase activity of S.aureus can be detected by supplementing the medium with egg yolk emulsion.
A possible S.aureus must be confirmed by the coagulase test. Also the organism should be subcultured to a less inhibitory medium not containing excess salt to avoid the possible interference of salt with coagulase testing or other diagnostic tests (e.g. Nutrient Broth) (M002) (9). Few strains of S.aureus may exhibit delayed mannitol fermentation. Negative results should therefore be re-incubated for an additional 24 hours before being discarded (9).
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (6,7).
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (1,12,13). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
1. A possible S.aureus must be confirmed by the coagulase test.
2. The organism should be subcultured to a less inhibitory medium not containing excess salt to avoid the possible interference of salt with coagulase testing or other diagnostic tests (e.g. Nutrient Broth) (M002) (9).
3. Few strains of S.aureus may exhibit delayed mannitol fermentation. Negative results should therefore be re-incubated for an additional 24 hours before being discarded (9).

Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.

Quality Control
Appearance
Light yellow to pink homogeneous free flowing powder
Gelling
Firm,comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Red coloured clear to slightly opalescent gel forms in Petri plates
Reaction
Reaction of 11.1% w/v aqueous solution at 25°C. pH : 7.4±0.2
pH
7.20-7.60
Cultural Response
Cultural characteristics observed after an incubation at 35-37°C for 18-72 hours. Recovery rate is considered as 100% for bacteria growth on Soybean Casein Digest Agar.

03/03/2022

Campylobacter Agar Base
Intended use
Recommended for the selective isolation of Campylobacter species from faecal, food and environmental specimens.
Composition**

Ingredients Gms / Litre
Proteose peptone 15.000
HML extract # 2.500
Yeast extract 5.000
Sodium chloride 5.000
Agar 12.000
Final pH ( at 25°C) 7.4±0.2
**Formula adjusted, standardized to suit performance parameters # Equivalent to Liver digest
Principle And Interpretation
Campylobacter species are ubiquitous in the environment inhabiting a wide variety of ecological niches (8). Infection with a
Campylobacter species is one of the most common causes of human bacterial gastroenteritis (8). Most species are found in animals (cattle, swine) and cause infertility and abortion (7). C. jejuni was originally isolated on a blood-containing media
with antibiotics (3). Skirrow described a selective medium for Campylobacter species consisting of Blood Agar Base No. 2 supplemented with horse blood and antibiotics (9). Subsequently, Bl**er et al isolated C.jejuni on Brucella Agar supplemented with sheep blood and four antibiotics (2). Later on, a fifth antibiotic, cephalothin was added to improve the selectivity of the medium by inhibition of accompanying faecal bacteria (12). Campylobacter Agar Base is recommended by
APHA for selective isolation of Campylobacter species (10).
Campylobacter Agar Base is well supplemented to support luxuriant growth of Campylobacter species. Osmotic equilibrium of the medium is maintained by sodium chloride. Blood serves as an additional source of nutrients including X-factor. The antibiotic supplements namely Bl**er-Wang (FD006) and Skirrow (FD008) markedly reduce the growth of normal enteric bacteria while enhancing the growth and recovery of C.jejuni from faecal specimens. Amphotericin B in Bl**er- Wang supplement greatly or completely inhibits growth of fungi. C.jejuni colonies appear non-haemolytic, flat and gray with an irregular edge or raised and round with a mucoid appearance. Some strains may appear tan or slightly pink. Swarming may be observed on moist surfaces. Incubation at 35-37°C may show a delayed growth of C.jejuni cultures. Incubating the plates at 42°C can fasten this.
The contaminated food sample (10 to 25 grams) is enriched in Campylobacter Enrichment Broth Base (M899 + FD042). The broth is incubated with agitation under a micro aerobic atmosphere for 16-18 hrs. The enrichment culture is then plated onto the selective media i.e. Campylobacter Agar Base ..
Type of specimen
Clinical samples - Faeces; Food and dairy samples; Environmental samples.
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (5,6).
For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (1,4,11). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
In vitro diagnostic use. Read the label before opening the container. Wear protective gloves/ protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
1. Due to nutritional variations, some strains may show poor growth.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Gelling
Firm, comparable with 1.2% Agar gel.
Colour and Clarity of prepared medium
Basal medium: Yellow coloured clear gel After addition of 5-7% v/v lysed blood: Reddish brown coloured opaque gel forms in Petri plates
Reaction
Reaction of 3.95% w/v aqueous solution at 25°C. pH : 7.4±0.2
pH 7.20-7.60

03/03/2022

EMB Agar
Intended Use:
Recommended for differential isolation of Gram-negative enteric bacilli from clinical and non-clinical specimens.
Composition**
Ingredients Gms / Litre
Peptone 10.000
Dipotassium hydrogen phosphate 2.000
Lactose 5.000
Saccharose (Sucrose) 5.000
Eosin - Y 0.400
Methylene blue 0.065
Agar 13.500
Final pH ( at 25°C) 7.2±0.2
**Formula adjusted, standardized to suit performance parameters

Principle And Interpretation
Eosin Methylene Blue (EMB) Agar was originally devised by Holt-Harris and Teague (2) and further modified by Levine (6). The above medium is a combination of the Levine and Holt-Harris and Teague formulae which contains peptone and phosphate as recommended by Levine and two carbohydrates as suggested by Holt-Harris and Teague. Methylene blue and Eosin-Y inhibit gram-positive bacteria to a limited degree. These dyes serve as differential indicators in response to the fermentation of carbohydrates. The ratio of eosin and methylene blue is adjusted approximately to 6:1. Sucrose is added to the medium as an alternative carbohydrate source for typically lactose-fermenting, gram-negative bacilli, which on occasion do not ferment lactose or do so slowly. The coliforms produce purplish black colonies due to taking up of methylene blue-eosin dye complex, when the pH drops. The dye complex is absorbed into the colony. Nonfermenters probably raise the pH of surrounding medium by oxidative deamination of protein, which solubilizes the methylene blue-eosin complex resulting in colourless colonies (3). Some strains of Salmonella and Shigella species do not grow in the presence of eosin and methylene blue. Further tests are required to confirm the isolates.
Peptone serves as source of carbon, nitrogen, and other essential growth nutrients. Lactose and sucrose are the sources of energy by being fermentable carbohydrates. Eosin-Y and methylene blue serve as differential indicators. Phosphate buffers the medium.
The test sample can be directly streaked on the medium plates. Inoculated plates should be incubated, protected from light. However standard procedures should be followed to obtain isolated colonies. A non-selective medium should be inoculated in conjunction with EMB Agar. Confirmatory tests should be further carried out for identification of isolated colonies.
Type of specimen
Clinical samples- Faecal samples, Food samples, Water samples
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (4,5). For food samples, follow appropriate techniques for sample collection and processing as per guidelines (7).
For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards.(1) After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture.
Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations :
1. Individual organisms differ in their growth requirement and may show variable growth patterns on the medium.
2. Each lot of the medium has been tested for the organisms specified on the COA. It is recommended to users to validate the medium for any specific microorganism other than mentioned in the COA based on the user’s unique requirement.
3. Confirmatory tests should be further carried out for identification of isolated colonies.

Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.
Quality Control
Appearance
Light pink to purple homogeneous free flowing powder
Gelling
Firm, comparable with 1.35% Agar gel.
Colour and Clarity of prepared medium
Reddish purple coloured, opalescent gel with greenish cast and finely dispersed precipitate forms in Petri plates
Reaction
Reaction of 3.6% w/v aqueous solution at 25°C. pH : 7.2±0.2

pH
7.00-7.40
Cultural Response
Cultural characteristics observed after an incubation at 35 - 37°C for 18 - 24 hours

شركة سينتفك آي للتجهيزات العلمية

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