09/04/2026
In molecular biology experiments, the accurate detection of nucleic acid concentration and purity is a core prerequisite for the success of subsequent experiments such as PCR and gene sequencing. Excessively high concentrations may inhibit the reaction system, while excessively low concentrations can affect experimental sensitivity and even lead to direct experimental failure.
Currently, the mainstream methods for nucleic acid concentration detection are absorbance and fluorescence methods, with corresponding instruments including micro-spectrophotometers, fluorometers, and fluorescence microplate readers, respectively. Although they all output concentration values, their detection principles differ significantly, and they are suited to different application scenarios.
Today we'll take a comprehensive look at how to choose the right instruments for different scenarios and avoid common testing mistakes.
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