17/03/2024
Principle A highly selective blue nuclear stain, Harris’ hematoxylin, is combined with EA50
polychromic mixture, a subtle cytoplasmic stain which differentiates cyanophil cells from
eosinophil ones. The last ingredient is OG6 solution, which stains keratinized elements.
Method
1) Ethanol 95°, 2 minutes
2) Distilled water, 2 minutes
3) Harris Hematoxylin, 1 minute
4) Tap water, 5 minutes
5) Ethanol 95°, 15 seconds
6) OG 6, 2 minutes
7) Ethanol 95°, 15 seconds (twice)
8) EA 65, 5 minutes
9) Ethanol 95°, 15 seconds
10) Absolute Ethanol, 30 seconds (twice)
11) Xilene or Bio Clear, 2 minutes (twice)
https://altibiaworld.com/product/papanicolaou-og6-1l/
