
15/09/2022
Blood, Blood, Blood
How to haemolyse blood.
Ever had the lab ring you in the ward and say you need to rebleed Mr Jones, his sample was haemolysed?
Well me too!
Haemolytic blood most commonly occurs when these four things occur:
1. You took the blood through a small or bent needle.
Use a 21g (green hub) needle to take blood where possible. I know we get those patients with tiny weedy veins but you are doing no favours using a 23g or smaller needle. Never bend the needle- a rigid tube structure will narrow its intralumenal diameter whenever you bend it. As red blood cells are squeezed through the bend their flow is turbulent, some cells fracture and release the haem into the serum - haemolysis.
2. If you take blood from a cannula, put syringe directly onto the cannula hub. Avoid pulling a sample through the valve of a b**g. Again, just like a bent needle, many b**gs have a valve structure that causes turbulence to flow, fracturing the RBCs.
3. Use the smallest syringe necessary, and ease back the plunger at the rate of flow so as to avoid too much swirling of the blood under high vacuum. A well engorged vessel will almost fill a 5-10ml syringe under its own pressure. 20-30 ml syringes create more vacuum and thus risk damaging the blood.
4. Never never never shake the blood tubes. Blood is a delicate fragile medium that when shook, breaks- literally. Be gentle and rock the blood tube to mix the sample 7-8 time before setting it down to bag.
Bonus tip for those who in rural areas spin their blood. Never spin it fresh. The yellow top tube (SST) must be allowed to form a clot befor you spin. This takes 5-15 mins for most patients. Those on warfarin or DOACs might need a couple of extra mins, but a red lump needs to be visible before you put it in the spinner.
There is probably more tips you can share below but as a Cannulation and venepuncture teacher for 15 years, these 5 tips would be the highest yield.
Quick post .
Hope it helps:
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