05/01/2026
Due to overwhelming requests from injectors worldwide asking how to properly prepare PRF, we decided to create a more detailed step-by-step reel.
🔑 Important Points:
• PRF preparation alone takes ~30 minutes
• Safety principles are the same as HA fillers and must always be followed
• PRF is more viscous → requires more force to inject
• Larger needles or cannulas may be needed for smoother delivery
⸻
🧪 Bio-Filler (PRF) Preparation Protocol
1️⃣ Turn on the JoanLab tube warmer (≈12 minutes to reach 75°C)
2️⃣ Draw blood into sterile tubes without anticoagulants (no ACD, gel, or additives)
3️⃣ Centrifuge at 1800 RPM for 8 minutes
4️⃣ Extract the lower 2 cc (platelet-rich plasma) → heat at 75°C for 15 minutes, then cool in ice water for 2 minutes
5️⃣ Extract the upper 2 cc plasma → place in ice water for 2 minutes
6️⃣ Mix upper + lower fractions using a Luer-lock to Luer-lock (orange connector) to form Bio-Filler
7️⃣ Inject immediately using a cannula or needle
⚠️ Always aspirate before injecting
💡 Bio-Cooler can be a device or simply a glass of ice water
💡 Tube warmer is essential for controlled heating
⸻
🌍 We offer online & in-person PRF/Bio-Filler training worldwide
📩 DM us for details
⸻
CosmeticMedicine PRFTraining MedicalAesthetics SkinRejuvenation NaturalResults CollagenStimulation VancouverAesthetics RichmondBC DrSahih AestheticTraining