21/05/2026
How do you study complex receptor signaling without background noise interfering with your readouts? You start with a perfectly clean cellular background. 🧫✨
We recently sat down with Dr. Shixin Ye-Lehmann ( ) to discuss her team's work on the Hedgehog signaling pathway and the SMO receptor using synthetic biology. 🔬
To get clean readouts for their functional assays, they needed a precise SMO gene knockout in SK-OV-3 cells. They didn't just need a simple mutation; they needed a complete, homozygous deletion to ensure zero background interference. 🧬
Using a double-guide approach and single-cell isolation, successfully deleted the region between exons 2 and 6, delivering the verified homozygous knockout clones needed to move their research forward. 🚀
Read the full conversation and see how CellEDIT enables breakthrough synthetic biology research. Link in the first comment 👇