After practicing rural service in Ioannina he was admitted to practice the specialty of Gynecology-Obstetrics in the UK. During the specialisation he worked in 6 hospitals in England and Scotland, gaining experience in all areas of specialty such as obstetrics, high-risk pregnancy, modern laparoscopic and hysteroscopic surgery, obstetric and gynecological ultrasound, colposcopy and cervical pathology, infertility and IVF. Upon completing his specialty, Dr. Nikolopoulos worked for 3 years as a consultant in Great Britain and specialised in Laparoscopic and Hysteroscopic Surgery, as well as Infertility and IVF, dealing with the full spectrum of infertile couples’ care and modern methods of IVF and assisted reproduction. He has worked until 2010 at the “Embio” and “Diagnosis” IVF Units and, from 2010 until December of 2015, at the Assisted Reproduction Unit of the “IVF Athens Center”. He's current post is consultant in Hygeia IVF Embryogenesis. He also currently holds a private practice in Athens and is the Head of infertility and in vitro fertilization at the clinic of Kalamata City Hospital, under the auspices of Health IVF Embryogenesis.