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Medical Laboratory Technology, medical college, KOLKATA (2025)

20/06/2025

🌟 (Gell and Coombs Classification)

Type→ Name →Immune Component→Mechanism of Damage →Onset →Examples

I→ Immediate (Anaphylactic) IgE + Mast cells/Basophils Allergen cross-links IgE on mast cells → Histamine release Seconds to minutes Asthma, Anaphylaxis, Hay fever, Urticaria, Food allergy

II → Cytotoxic (Antibody-mediated) IgG/IgM + Complement or ADCC Antibodies bind to antigens on cells → Cell destruction Minutes to hours Hemolytic anemia, Goodpasture's, Rheumatic fever, GBS

III→ Immune Complex IgG/IgM + Antigen + Complement Immune complexes deposit in tissues → Inflammation Hours to days SLE, Post-streptococcal GN, Serum sickness, Arthus reaction

IV→ Delayed-type (Cell-mediated) T cells (CD4+/CD8+) Sensitized T cells release cytokines → Macrophage activation or cytotoxicity 48–72 hours Tuberculosis, Contact dermatitis, Type 1 diabetes, MS

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🔍 Detailed Explanation of Each Type

✅ Type I – Immediate Hypersensitivity

Main antibody: IgE
Cells involved: Mast cells, basophils
Pathway: First exposure sensitizes → IgE binds to mast cells → Second exposure causes degranulation → Histamine release
Clinical signs: Swelling, itching, bronchospasm, hypotension (in anaphylaxis)

✅ Type II – Cytotoxic Hypersensitivity

Main antibodies: IgG, IgM
Pathway: Antibodies bind to cell surface antigens → Activate complement or ADCC → Cell lysis or opsonization
Key feature: Destruction of specific cells

Examples:
Autoimmune hemolytic anemia
Myasthenia gravis
Graves' disease (also has stimulatory antibodies)

✅ Type III – Immune Complex-Mediated Hypersensitivity

Main components: Antigen–antibody (IgG/IgM) complexes + complement
Pathway: Complexes deposit in small vessels → Complement activation → Neutrophil recruitment → Inflammation & tissue damage

Examples:
Systemic lupus erythematosus (SLE)
Post-streptococcal glomerulonephritis
Serum sickness
Arthus reaction (localized)

✅ Type IV – Delayed-type Hypersensitivity

Main cells: T lymphocytes (CD4+ helper T cells, CD8+ cytotoxic T cells)
Pathway: Antigen-presenting cells activate T cells → Cytokine release or direct killing → Tissue damage
Not antibody mediated

Examples:
Tuberculin skin test (Mantoux test)
Contact dermatitis (e.g., poison ivy, nickel)
Type 1 diabetes mellitus
Multiple sclerosis

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📌 Mnemonic to Remember Types:

"ACID"

A – Type I: Anaphylactic (IgE)

C – Type II: Cytotoxic (IgG/IgM)

I – Type III: Immune complex

D – Type IV: Delayed (T-cell)

゚viralシfypシ゚viralシalシ゚viralシ

06/06/2025

゚viralシ゚viralシfypシ゚viralシalシ

02/06/2025

is a combination vaccine that protects against five major potentially deadly childhood diseases:

1.
2. (Whooping Cough)
3.
4.
5. (Hib)

Key Points:
Formulation: It combines DPT (Diphtheria, Pertussis, Tetanus), Hepatitis B, and Hib into a single shot.

Schedule in India (as per the Universal Immunization Programme - UIP):

Pentavalent Vaccine Schedule (3 doses):
1. 6 weeks – Pentavalent-1
2. 10 weeks – Pentavalent-2
3. 14 weeks – Pentavalent-3

Follow-up Boosters (2 more doses):
4. 16–24 months – DPT + Hib booster (Not pentavalent, but protects against the same diseases except Hep B)
5. 5–6 years – DPT booster (Continued protection against Diphtheria, Pertussis, and Tetanus)

So:
3 doses of Pentavalent vaccine directly.
2 booster doses for extended protection (DPT & Hib).

_________________
Dose No. Age . Vaccine Given. Diseases Covered

1. 6 weeks Pentavalent-1 Diphtheria, Pertussis, Tetanus, Hep B, Hib
2. 10 weeks Pentavalent-2 Diphtheria, Pertussis, Tetanus, Hep B, Hib
3. 14 weeks Pentavalent-3 Diphtheria, Pertussis, Tetanus, Hep B, Hib
4. (Booster) 16–24 months DPT + Hib Booster Diphtheria, Pertussis, Tetanus, Hib
5. (Booster) 5–6 years DPT Booster Diphtheria, Pertussis, Tetanus

> ✅ Note: The Hepatitis B birth dose is a separate injection given within 24 hours of birth and is not part of the pentavalent doses.
__________________
Benefits:
Reduces the number of injections a child receives.
Improves vaccination coverage and compliance.
Protects against serious diseases that can cause death or long-term disabilities.

Side Effects:

Mild fever
Pain/swelling at the injection site
Irritability or drowsiness
Severe allergic reactions are rare but require immediate medical attention.
゚viralシ

31/05/2025

゚viralシ Medical Laboratory Technology

23/05/2025

(IgG) is the most abundant type of antibody in the human body and plays a crucial role in the immune system. Here are detailed insights:

Basic Overview:

Full Name: Immunoglobulin G
Type: Monomeric antibody (Y-shaped)
Molecular Weight: ~150 kDa
Serum Concentration: 7–16 g/L (most abundant immunoglobulin in circulation, ~75–80% of serum antibodies)

Structure:
Composed of:
2 heavy chains (γ chains)
2 light chains (κ or λ)

Contains:
Fab regions (antigen-binding)
Fc region (binds to Fc receptors on immune cells and complement proteins)

Subclasses of IgG:
There are four subclasses, differing in structure and function:
1. IgG1 – 60–70% of total IgG; responds to protein antigens (e.g., viruses, toxins)
2. IgG2 – 20–25%; targets polysaccharide antigens (e.g., bacterial capsules)
3. IgG3 – 5–10%; effective in complement activation
4. IgG4 – ~4%; involved in chronic antigen exposure, least inflammatory

Functions:
Neutralization: Binds and neutralizes toxins, viruses, and bacteria
Opsonization: Marks pathogens for destruction by phagocytes
Complement Activation: IgG1, IgG3 initiate classical complement pathway
Antibody-Dependent Cellular Cytotoxicity (ADCC): Activates NK cells via Fc receptors
Immune Memory: Produced during secondary immune response
Placental Transfer: Only Ig class that crosses the placenta to provide passive immunity to the fetus

Clinical Significance:

Elevated IgG:
Chronic infections (e.g., tuberculosis)
Autoimmune diseases (e.g., lupus, rheumatoid arthritis)
Multiple myeloma
Chronic liver disease

Decreased IgG:
Immunodeficiencies (e.g., Common Variable Immunodeficiency – CVID)
Protein-losing conditions (e.g., nephrotic syndrome)
Certain leukemias

IgG Testing:
Quantitative IgG test: Measures serum IgG levels
IgG subclass test: Analyzes each subclass separately
Specific IgG antibodies: Used to detect past exposure or immunity to specific pathogens (e.g., Hepatitis B, COVID-19)

Therapeutic Uses:
IVIG (Intravenous Immunoglobulin):
Prepared from pooled donor plasma
Used to treat autoimmune disorders, immunodeficiencies, and inflammatory diseases

゚viralシ

22/05/2025

:

1. Basic Structure:
Immunoglobulins (Ig), also known as antibodies, are glycoproteins produced by B cells (plasma cells) in response to antigens. Each immunoglobulin molecule consists of 4 polypeptide chains:
- 2 identical heavy chains (H chains)
- 2 identical light chains (L chains) — either kappa (κ) or lambda (λ)

The structure has distinct regions:
- Variable (V) region: Binds to specific antigens
- Constant (C) region: Determines class and function

The Y-shaped molecule has:
- Top arms (Fab region) bind to antigens
- Bottom stem (Fc region) interacts with immune cells

2. Types (Classes) of Immunoglobulins:
Please check comment

3. Functions of Immunoglobulins:
*. Neutralization – Block pathogens or toxins from entering cells.
*. Opsonization – Enhance phagocytosis by marking pathogens.
*. Complement activation – Initiates classical pathway.
*. Antibody-dependent cell-mediated cytotoxicity (ADCC) – Directs immune cells to destroy target cells.
*. Agglutination – Clumps antigens together for easier removal.
*. Precipitation – Soluble antigens are made insoluble for removal.

4. Structure Variants
- Monomer: IgG, IgE, IgD
- Dimer: IgA (with J chain and secretory component)
- Pentamer: IgM (with J chain)

5. Clinical Significance
- IgG: Measured in immunity tests (e.g., post-vaccination titers), autoimmune diseases.
- IgA: Deficiency linked to mucosal infections.
- IgM: Indicates recent infection.
- IgE: Elevated in allergies and parasitic infections.
- IgD: Role still being researched; marker of B cell maturation.

________________________

1. IgM:

First antibody produced during an immune response.
Acts like the "first wave of soldiers" for early defense.

2. IgA:
Provides protection at surface barriers (e.g., gut, respiratory tract).
Acts like a surveillance system in mucous membranes.

3. IgE:
Involved in allergic reactions.
High levels often indicate an allergic response.

4. IgD:
Found in small amounts in the blood.
Its function is the least understood.

5. IgG:
Most abundant antibody.
Provides long-term protection by fighting bacterial and viral infections.
゚viralシ

20/05/2025

゚viralシ

18/05/2025

, including types, causes, symptoms, and lab diagnosis:

What is Anemia?
Anemia is a condition characterized by a decrease in the total amount of red blood cells (RBCs) or hemoglobin (Hb) in the blood, leading to reduced oxygen-carrying capacity.

Classification of Anemia

1. Based on RBC Size (MCV - Mean Corpuscular Volume):

Microcytic anemia (MCV < 80 fL)
Iron Deficiency anemia
Thalassemia
Anemia of chronic disease (occasionally)
Sideroblastic anemia

Normocytic anemia (MCV 80–100 fL)
Acute blood loss
Hemolytic anemia
Anemia of chronic disease
Aplastic anemia

Macrocytic anemia (MCV > 100 fL)
Vitamin B12 deficiency
Folate deficiency
Alcoholism
Liver disease
Hypothyroidism
Myelodysplastic syndrome

Etiological Classification:
Blood loss: Acute (trauma), Chronic (GI bleeding, menorrhagia)

Decreased RBC production:
Nutritional deficiencies (Iron, B12, folate)
Bone marrow failure
Chronic diseases

Increased RBC destruction:
Hemolytic anemia (hereditary: spherocytosis, G6PD; acquired: autoimmune)

Common Symptoms:
Fatigue, weakness
Pallor (skin, conjunctiva)
Dyspnea
Palpitations
Dizziness
Pica (in iron deficiency)
Neurological symptoms (in B12 deficiency)

Lab Diagnosis of Anemia

1. Complete Blood Count (CBC)

Hemoglobin (Hb): Low in anemia

Hematocrit (Hct): Usually reduced

RBC Count: Decreased

MCV: Helps classify anemia (micro, normo, macrocytic)

MCH/MCHC: Assess hemoglobin content per RBC

RDW: Red cell distribution width – increased in mixed or iron-deficiency anemia

2. Peripheral Blood Smear

Morphology of RBCs:

Microcytic, hypochromic (iron deficiency)

Macrocytic (megaloblastic)

Spherocytes, schistocytes (hemolysis)

Target cells (thalassemia)

Hypersegmented neutrophils (B12/folate deficiency)

3. Reticulocyte Count

Indicates bone marrow response:

Low in production defects

High in hemolysis or blood loss

4. Iron Studies

Serum iron: Low in iron deficiency

TIBC (Total iron-binding capacity): Increased in iron deficiency

Ferritin: Low in iron deficiency; high in anemia of chronic disease

Transferrin saturation: Low in iron deficiency

5. Vitamin B12 & Folate Levels

Low in megaloblastic anemia

6. Hemolysis Workup

LDH: Increased

Indirect bilirubin: Increased

Haptoglobin: Decreased

Coombs test: Positive in autoimmune hemolytic anemia

7. Bone Marrow Examination

If diagnosis unclear or for aplastic anemia, leukemia, etc.

Erythroid hyperplasia in hemolysis or megaloblastic anemia

8. Special Tests

Hb electrophoresis: Thalassemia, sickle cell anemia

G6PD assay: G6PD deficiency

Osmotic fragility test: Hereditary spherocytosis

Treatment Overview

Iron deficiency: Oral or IV iron

B12/Folate deficiency: Supplements

Hemolytic anemia: Treat underlying cause, steroids, transfusions

Chronic disease anemia: Manage underlying disease

Severe anemia: Blood transfusion if Hb critically low

16/05/2025

G6PD stands for Glucose-6-Phosphate Dehydrogenase, an enzyme found in red blood cells. It plays a crucial role in protecting red blood cells from damage and premature destruction.

Key Points about G6PD:

1. Function:

G6PD is essential for the pentose phosphate pathway, which helps produce NADPH, a molecule that protects red blood cells from oxidative stress.

2. G6PD Deficiency:

A genetic condition where the body doesn't produce enough of the enzyme.

Most common in males (X-linked inheritance).

Can lead to hemolytic anemia (destruction of red blood cells), especially when exposed to certain triggers.

3. Common Triggers:

Certain medications (e.g., sulfa drugs, antimalarials)

Infections

Fava beans (leading to “favism”)

4. Symptoms of G6PD Deficiency:

Jaundice

Dark urine

Fatigue

Rapid heart rate

Shortness of breath

5. Diagnosis:

Blood tests to measure G6PD enzyme activity.

Often included in newborn screening in some countries.

6. Management:

Avoidance of known triggers.

Supportive care during hemolytic episodes (hydration, oxygen, sometimes blood transfusion).

12/05/2025

in Laboratory Diagnosis:

1. Classification of Culture Media:

A. Based on Consistency:

*Type Description:
Contains agar (1.5–2%);
used for colony isolation (e.g., Nutrient agar, MacConkey agar)
Contains less agar (0.2–0.5%);
used for motility testing
(Broth) No agar;
used for growing large numbers of bacteria (e.g., Nutrient broth, TSB)

B. Based on Function:

Type - Function - Examples

- Support growth of non-fastidious organisms - Nutrient agar, Nutrient broth

- Contain additional nutrients for fastidious organisms - Blood agar, Chocolate agar

- Inhibit growth of certain microbes while allowing others - MacConkey agar, TCBS

- Distinguish between species based on metabolic traits - MacConkey agar, XLD

- Promote growth of specific pathogens from mixed cultures - Selenite F broth, APW

- Maintain viability of organisms during transport - Stuart’s, Amies, Cary-Blair

- Support growth of anaerobic organisms - Robertson's cooked meat medium

2. Common Culture Media and Their Uses:

Medium -Type - Contents - Used For

- Basic - Peptone, beef extract, agar - General purpose

- Enriched, differential - 5 to 10% sheep blood - Hemolytic pattern (alpha, beta, gamma)

- Enriched - Heated blood, nutrients - Fastidious organisms

- Selective, differential - Bile salts, lactose, neutral red - Gram-negative enterics

- Selective, differential - Xylose, lysine, deoxycholate - Salmonella, Shigella

- Selective - Thiosulfate, citrate, bile salts, sucrose - Vibrio species

- Selective - Egg-based, malachite green - Mycobacterium tuberculosis

- Selective - Peptone, glucose, low pH - Fungi and yeast

- Selective - Blood agar with antibiotics - Campylobacter jejuni

- Enriched - Charcoal, yeast extract - Legionella

- Enrichment - Sodium selenite - Salmonella from f***s

- Enrichment - Peptone, high pH - Vibrio cholerae enrichment

- Enrichment, anaerobic Thioglycollate, resazurin - Anaerobes

3. Specialized Media for Certain Bacteria:

Bacterium - Preferred Medium

-Lowenstein-Jensen
- Chocolate agar, Thayer-Martin
- Chocolate agar
. - XLD, Selenite F broth
- TCBS, Alkaline Peptone Water
- Loeffler’s serum slope, Tinsdale agar
Clostridium_spp.- Robertson's cooked meat medium
Legionella pneumophila - BCYE agar
Fungi - Sabouraud Dextrose Agar (SDA)

4. Media Preparation Tips:

• Always sterilize media using autoclaving (121°C, 15 psi, 15–20 min) unless heat-sensitive.
• Pour plates under aseptic conditions.
• Store media at 2–8°C if not used immediately.
• Observe expiry and avoid desiccated or contaminated plates.

゚viralシfypシ゚viralシalシ

07/05/2025

(White Blood Cell) Total Count is a common hematology test performed in the laboratory to measure the total number of white blood cells in a blood sample. It helps assess the immune system and detect infections, inflammation, allergies, blood cancers, and other medical conditions.

Purpose of WBC Total Count:
Detect infections (elevated WBC)
Monitor bone marrow function
Detect leukopenia (low WBC) or leukocytosis (high WBC)
Aid diagnosis of diseases like leukemia or immune disorders

Normal Reference Range:
Adults: 4,000 – 11,000 cells/µL (4.0 – 11.0 × 10⁹/L)
Children: Slightly higher ranges depending on age

Sample Required:
Anticoagulated venous blood (commonly in an EDTA tube)

Methods of Estimation:
1. Manual Method (Hemocytometer/Neubauer Chamber):
Blood is diluted (usually with Turk’s fluid or acetic acid solution).
Count is done under a microscope using a hemocytometer.

Formula used:

WBC Count - Nuber of cels counted x Dilution factor x 10 /Number of squares counted

2. Automated Hematology Analyzer:
Most commonly used in modern labs.
Rapid and more accurate.
Uses flow cytometry or impedance methods.

Interpreting Results:

Condition. WBC Count. Possible Causes

Leukocytosis 11,000/uL. Infection,inflammation,leukemia
Leukopenia.

Medical Laboratory Technology

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