15/10/2025
What is the meaning of Primary Wavelength & Secondary wavelength
➡️ λ₁ is the main wavelength used to measure analyte absorbance.
➡️ λ₂ is reference wavelength used to correct interference (turbidity, sample colour etc.)
➡️ E.g. Bilirubin T&D
Primary Wavelength (λ₁):
546 nm - Measures the true absorbance of the purple azobilirubin complex.
Secondary Wavelength (λ₂):
600 nm - Corrects background interference (due to turbidity or sample colour).
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