
25/07/2025
YMC presents a new application of AEX-MS (Anion Exchange Chromatography with Mass Spectrometry) for detailed charge variant analysis of IgG4-based monoclonal antibodies.
By combining BioPro IEX QF columns with PNGase F-mediated deglycosylation, the method enables precise separation of glycosylation variants, deamidated forms, and C-terminal Lys species. It also supports Fc fragment analysis at different temperatures, helping identify conformational isomers and improving resolution at lower pI values.
This approach offers a robust solution for critical quality attribute (CQA) monitoring and enhances confidence in mAb profiling and characterization.
Nagesh Kumar Kandukuri