01/07/2025
Urine MCS (Microscopy, Culture & Sensitivity) test
1. Objective
The objective of the test was to detect the presence of microorganisms in urine and determine their antibiotic sensitivity, helping diagnose urinary tract infections (UTIs).
2. Principle
The Urine MCS test involved three steps:
Microscopy detected pus cells, RBCs, casts, crystals, and bacteria.
Culture isolated and identified bacteria by inoculating urine onto culture media.
Sensitivity determined which antibiotics effectively inhibited the bacterial growth.
3. Materials
Midstream clean-catch urine sample
Sterile container
Culture media (CLED, MacConkey, Blood agar)
Gram stain reagents
Microscope
Incubator
Antibiotic discs (for sensitivity testing)
4. Procedure
A. Microscopy
1. A drop of well-mixed, uncentrifuged urine was examined under a microscope.
2. Presence of pus cells, RBCs, crystals, and bacteria was noted.
B. Culture
1. Urine was inoculated onto culture media using a calibrated loop.
2. Plates were incubated at 37°C for 24 hours.
3. Bacterial colonies were identified by morphology and biochemical tests.
C. Sensitivity
1. Isolated bacteria were spread on Mueller-Hinton agar.
2. Antibiotic discs were placed.
3. Plates were incubated, and zones of inhibition were measured.
5. Result
Microscopy:
Pus cells: >10/HPF (indicative of infection)
Bacteria: Present
Culture:
Significant growth (>10⁵ CFU/mL) of E. coli
Sensitivity:
Sensitive to: Nitrofurantoin, Ciprofloxacin
Resistant to: Ampicillin
6. Uses
Diagnosed UTIs and identified causative organisms
Guided targeted antibiotic therapy
Evaluated treatment effectiveness and recurrent infections
7. Conclusion
Urine MCS was essential in diagnosing urinary tract infections and determining the appropriate antibiotic, helping reduce the risk of resistance and recurrence.