TOMOCUBE

29/08/2025

This live-cell video showcases two remarkable cellular behaviors: 𝘁𝗵𝗲 𝗿𝗼𝘁𝗮𝘁𝗶𝗼𝗻 𝗼𝗳 𝘁𝗵𝗲 𝗻𝘂𝗰𝗹𝗲𝘂𝘀 and active 𝗽𝗶𝗻𝗼𝗰𝘆𝘁𝗼𝘀𝗶𝘀, where the cell "drinks" its surrounding fluid by forming small vesicles.

Captured using high-resolution label-free imaging, the footage reveals the complex and dynamic nature of intracellular movement and nutrient uptake in real time.

Click to explore how is pushing the boundary of cell biology:
https://tomocube.com/application/cell_biology

27/08/2025

🧠✨ New insights into bipolar disorder metabolism

Congratulations to Prof. Jinju Han and colleagues at KAIST and collaborators on their recent publication in Molecular Psychiatry! The study revealed 𝗵𝗼𝘄 𝗮𝘀𝘁𝗿𝗼𝗰𝘆𝘁𝗲𝘀 𝗳𝗿𝗼𝗺 𝗯𝗶𝗽𝗼𝗹𝗮𝗿 𝗱𝗶𝘀𝗼𝗿𝗱𝗲𝗿 𝗽𝗮𝘁𝗶𝗲𝗻𝘁𝘀 𝗲𝘅𝗵𝗶𝗯𝗶𝘁 𝗺𝗶𝘁𝗼𝗰𝗵𝗼𝗻𝗱𝗿𝗶𝗮𝗹 𝗱𝘆𝘀𝗳𝘂𝗻𝗰𝘁𝗶𝗼𝗻 𝗮𝗻𝗱 𝗮𝗯𝗻𝗼𝗿𝗺𝗮𝗹 𝗹𝗶𝗽𝗶𝗱 𝗱𝗿𝗼𝗽𝗹𝗲𝘁 𝗮𝗰𝗰𝘂𝗺𝘂𝗹𝗮𝘁𝗶𝗼𝗻, with lithium correcting this only in responders.

To reveal these lipid droplet changes, researchers turned to Tomocube’s holotomography system, enabling 𝗹𝗮𝗯𝗲𝗹-𝗳𝗿𝗲𝗲, 𝟯𝗗 𝘃𝗶𝘀𝘂𝗮𝗹𝗶𝘇𝗮𝘁𝗶𝗼𝗻 𝗮𝗻𝗱 𝗾𝘂𝗮𝗻𝘁𝗶𝗳𝗶𝗰𝗮𝘁𝗶𝗼𝗻 𝗼𝗳 𝗹𝗶𝘃𝗲 𝗮𝘀𝘁𝗿𝗼𝗰𝘆𝘁𝗲𝘀. This approach solved a key challenge: tracking lipid metabolism without disrupting cell physiology.

🔍 The findings highlight astrocyte-specific metabolic dysfunction as a hallmark of bipolar disorder, and position lipid droplet imaging as a potential tool for new drug discovery.

Read the article here: https://www.nature.com/articles/s41380-025-03176-w

25/08/2025

📍 — A great start at 𝗞-𝗕𝗿𝗮𝗶𝗻 𝟮𝟬𝟮𝟱 & 𝘁𝗵𝗲 𝟯𝗿𝗱 𝗖𝗝𝗞 𝗡𝗲𝘂𝗿𝗼𝘀𝗰𝗶𝗲𝗻𝗰𝗲 𝗠𝗲𝗲𝘁𝗶𝗻𝗴 with exciting discussions at our booth and inspiring poster presentations from our team!

We’re looking forward to more exciting discussions in the days ahead — come visit us if you’re here!

22/08/2025

🎉 We’re excited to share our new collaborative publication with researchers from KAIST, Chungnam National University Hospital, and the Institute for Basic Science!

This work provides a 𝗰𝗼𝗺𝗽𝗹𝗲𝘁𝗲 𝘄𝗼𝗿𝗸𝗳𝗹𝗼𝘄 — from organoid preparation, imaging acquisition, to computational analysis — enabling researchers to monitor 𝗿𝗲𝗮𝗹-𝘁𝗶𝗺𝗲 𝘀𝘁𝗿𝘂𝗰𝘁𝘂𝗿𝗮𝗹 𝗱𝘆𝗻𝗮𝗺𝗶𝗰𝘀 𝗮𝗻𝗱 𝗱𝗿𝘂𝗴 𝗿𝗲𝘀𝗽𝗼𝗻𝘀𝗲𝘀 in living organoids with unmatched clarity.

✨ 𝗪𝗵𝘆 𝗵𝗼𝗹𝗼𝘁𝗼𝗺𝗼𝗴𝗿𝗮𝗽𝗵𝘆 𝗺𝗮𝘁𝘁𝗲𝗿𝘀 𝗶𝗻 𝗼𝗿𝗴𝗮𝗻𝗼𝗶𝗱 𝗿𝗲𝘀𝗲𝗮𝗿𝗰𝗵:

✅ 𝗟𝗮𝗯𝗲𝗹-𝗳𝗿𝗲𝗲 & 𝗻𝗼𝗻-𝗶𝗻𝘃𝗮𝘀𝗶𝘃𝗲: preserves organoid integrity, avoids phototoxicity, and eliminates the need for staining or genetic labeling.

✅ 𝟯𝗗 𝘃𝗶𝘀𝘂𝗮𝗹𝗶𝘇𝗮𝘁𝗶𝗼𝗻: fully captures the intricate architecture of organoids in their true three-dimensional form.

✅ 𝗥𝗲𝗮𝗹-𝘁𝗶𝗺𝗲 𝗱𝘆𝗻𝗮𝗺𝗶𝗰𝘀: continuous live monitoring reveals transient cellular changes missed by conventional endpoint analyses.

✅ 𝗤𝘂𝗮𝗻𝘁𝗶𝘁𝗮𝘁𝗶𝘃𝗲 𝗶𝗻𝘀𝗶𝗴𝗵𝘁𝘀: refractive index-based imaging allows measurement of biophysical properties such as volume, protein density, and total protein content.

✅ 𝗦𝗰𝗮𝗹𝗮𝗯𝗹𝗲 𝗮𝗻𝗮𝗹𝘆𝘀𝗶𝘀: integrated machine learning-driven segmentation and quantitative analysis ensure unbiased, reproducible, and high-throughput quantification.

This protocol sets a new standard for organoid imaging, accelerating advances in regenerative medicine, disease modeling, and pharmaceutical screening.

The publication is 𝗽𝗮𝗰𝗸𝗲𝗱 𝘄𝗶𝘁𝗵 𝘃𝗮𝗹𝘂𝗮𝗯𝗹𝗲 𝗳𝗶𝗴𝘂𝗿𝗲𝘀 𝗮𝗻𝗱 𝗱𝗲𝘁𝗮𝗶𝗹𝗲𝗱 𝗱𝗮𝘁𝗮 that illustrate the full methodology and results. We invite you to explore the paper to see the complete workflow and findings in depth:

📖 https://app.jove.com/t/68529/label-free-high-resolution-3d-imaging-machine-learning-analysis

Korea Advanced Institute of Science and Technology (KAIST). We present a step-by-step protocol for high-resolution, label-free, and three-dimensional imaging of organoids using low-coherence holotomography. This protocol details organoid culture preparation, imaging acquisition, and computational im...

20/08/2025

👏 Congratulations to the researchers from Chungnam National University, Korea, on their new publication uncovering a novel host defense mechanism against .

The study demonstrates that endogenous hepcidin, induced by T cells activated with the Mycobacterium tuberculosis (Mtb) protein Rv1876, localizes to bacteria-containing phagosomes inside macrophages and directly interacts with Mtb, substantially inhibiting its growth — highlighting a promising target for host-directed therapies.

The Tomocube HT-X1 imaging system was used to investigate this 𝗧 𝗰𝗲𝗹𝗹-𝗱𝗿𝗶𝘃𝗲𝗻, 𝗺𝗮𝗰𝗿𝗼𝗽𝗵𝗮𝗴𝗲-𝗺𝗲𝗱𝗶𝗮𝘁𝗲𝗱, 𝗵𝗲𝗽𝗰𝗶𝗱𝗶𝗻-𝗱𝗲𝗽𝗲𝗻𝗱𝗲𝗻𝘁 𝗸𝗶𝗹𝗹𝗶𝗻𝗴 𝗺𝗲𝗰𝗵𝗮𝗻𝗶𝘀𝗺, enabling label-free 3D visualization of macrophage morphology combined with immunofluorescence imaging to reveal hepcidin localization adjacent to Mtb-containing phagosomes.

Holotomography was instrumental in confirming that Rv1876–T cell-induced hepcidin directly inhibits bacterial growth within macrophages, providing new insights into the fight against one of the world’s most persistent infectious diseases.

📖 Read the full publication here: https://www.tandfonline.com/doi/full/10.1080/22221751.2025.2539192

Tuberculosis (TB) is one of the most prevalent infectious diseases worldwide. However, few molecules related to bacterial killing within T cell-induced macrophages are known; therefore, elucidating...

18/08/2025

This August, Tomocube, Inc. is connecting with the scientific community at two upcoming events in Korea:

📅 August 22 – 2025 Annual Meeting of the Korean Society for Stem Cell Research
• Industry Session Presentation
• Poster Presentation

📅 August 24 – The 28th Annual Meeting of KSBNS K-Brain 2025 & The 3rd CJK Neuroscience Meeting
• Exhibition
• Poster Presentation

From stem cell research to neuroscience, we look forward to sharing how holotomography is advancing label-free 3D imaging across disciplines.

👉 If you’re attending, come meet our team and see our technology in action!

11/08/2025

Mike G. Crump, our Sales Director at Tomocube USA, returns with a deep dive into 𝗵𝗼𝘄 𝗵𝗼𝗹𝗼𝘁𝗼𝗺𝗼𝗴𝗿𝗮𝗽𝗵𝘆 𝗲𝗺𝗽𝗼𝘄𝗲𝗿𝘀 𝗺𝗶𝘁𝗼𝗰𝗵𝗼𝗻𝗱𝗿𝗶𝗮𝗹 𝗿𝗲𝘀𝗲𝗮𝗿𝗰𝗵.

From the core principle of label-free 3D imaging to capturing mitochondrial fission and fusion in action, Mike walks you through visualization, time-lapse observation, and quantitative measurement—all in a single, non-invasive workflow.

Whether you're studying mitochondrial dynamics or exploring new cellular insights, see how holotomography brings your research into sharper focus.

06/08/2025

💡 New publication reveals mechanism underlying fungal phytopathogenicity during host colonization!

Researchers from Japan and Spain uncovered that Fusarium oxysporum relies on two independent yet complementary morphogenetic processes to grow through microspaces that mimic the tight gaps between plant cells. This ability, driven by filamentous hyphae, is essential for successful colonization and virulence.

🔬 Tomocube’s HT-X1 holotomography enabled label-free 3D live imaging of fungal hyphae inside cross-shaped microfluidic channels, capturing real-time structural changes and refractive index shifts in both wild-type and mutant strains. This application highlights holotomography’s unique strength in visualizing cellular dynamic processes within complex microenvironments like microfluidic devices and live infection models.

👉 Go to the article: https://journals.asm.org/doi/10.1128/mbio.01184-25

This study highlights the critical role of hyphal plasticity and cell wall remodeling in the pathogenicity of Fusarium oxysporum, a major plant pathogen affecting over a hundred crops. The ability of fungal hyphae to traverse narrow plant tissue spaces, ...

30/07/2025

🔬 From full view to fine detail

A image of a liver organoid, acquired using tile imaging and shown as a maximum intensity projection.

On the right, a zoom-in cut reveals intricate subcellular structures, including cell membranes, nuclei, nucleoli, mitochondria, and vacuoles, all captured label-free and in 3D.

📷 Imaged with the Tomocube HT-X1 Holotomography System.

Explore the product: https://tomocube.com/products/htx1

28/07/2025

We’re proud to share exciting news: 𝗧𝗼𝗺𝗼𝗰𝘂𝗯𝗲’𝘀 𝗛𝗧-𝗫𝟭 𝗣𝗹𝘂𝘀 has been named one of the 𝗧𝗼𝗽 𝟭𝟬 𝗠𝗶𝗰𝗿𝗼𝘀𝗰𝗼𝗽𝘆 𝗜𝗻𝗻𝗼𝘃𝗮𝘁𝗶𝗼𝗻𝘀 in the 𝟮𝟬𝟮𝟱 𝗜𝗻𝗻𝗼𝘃𝗮𝘁𝗶𝗼𝗻 𝗔𝘄𝗮𝗿𝗱𝘀 by 𝘔𝘪𝘤𝘳𝘰𝘴𝘤𝘰𝘱𝘺 𝘛𝘰𝘥𝘢𝘺, the official publication of the Microscopy Society of America (Official).

Launched in 2024, the HT-X1 Plus is our most advanced holotomography system, offering increased sensitivity, higher throughput, and upgraded correlative imaging capabilities — all optimized for automated imaging and analysis workflows.

🔗 https://tomocube.com/products/htx1_plus

With the ability to capture both dynamic cellular activity and fine structural details, even in challenging samples such as organoids and thick tissue sections, the HT-X1 Plus sets a new benchmark for live imaging systems.

This award not only recognizes the innovation behind the HT-X1 Plus but also affirms its real-world impact across cell biology, immunology, oncology, and tissue research. A heartfelt thank you to Microscopy Today and the Microscopy Society of America (MSA) for this recognition!

25/07/2025

🧫𝗙𝗿𝗼𝗺 𝗖𝗲𝗹𝗹 𝗔𝗴𝗴𝗿𝗲𝗴𝗮𝘁𝗲𝘀 𝘁𝗼 𝗙𝘂𝗻𝗰𝘁𝗶𝗼𝗻𝗮𝗹 𝟯𝗗 𝗦𝘁𝗿𝘂𝗰𝘁𝘂𝗿𝗲𝘀

One of the most fascinating aspects of organoids is their ability to self-organize. When stem cells are placed in a supportive 3D environment and given the right biochemical cues, they spontaneously arrange themselves into miniature, organ-like structures.

This captivating timelapse shows the transformation of a mouse liver cell aggregate into a fully formed organoid over 72 hours, compressed into just 40 seconds.

21/07/2025

✨ 𝗪𝗵𝗮𝘁 𝗵𝗮𝗽𝗽𝗲𝗻𝘀 𝘄𝗵𝗲𝗻 𝗽𝗮𝘁𝗶𝗲𝗻𝘁-𝗱𝗲𝗿𝗶𝘃𝗲𝗱 𝗹𝘂𝗻𝗴 𝗰𝗮𝗻𝗰𝗲𝗿 𝗼𝗿𝗴𝗮𝗻𝗼𝗶𝗱𝘀 𝗮𝗿𝗲 𝗰𝗼𝗰𝘂𝗹𝘁𝘂𝗿𝗲𝗱 𝘄𝗶𝘁𝗵 𝗧 𝗰𝗲𝗹𝗹𝘀?

This video captures three timelapses showing the immune response in action — from T cell infiltration to targeted attack and cancer organoid destruction.

Watch now for a vivid look into how immune cells recognize and eliminate tumor cells in a 3D coculture environment.