MLS

31/01/2026

31/01/2026

Sterilisation

31/01/2026

BSL

31/01/2026

BIOSAFETY LEVELS.

30/09/2024

*SHINE YOUR LIGHT*

How do you feel when you don't get the reward, recognition or credit for your hard work? Bad?

I feel that way regularly as a medical laboratory professional. I and millions of others like me work tirelessly behind the scenes to ensure accurate diagnoses, yet we receive little to no recognition for the crucial role we play. Maybe we are invisible because our work happens in the lab, away from the public eye.

But while doctors and nurses are at the forefront of patient care, the lab scientists provide the vital data needed to make informed decisions. We analyze blood samples, diagnose infections, monitor diseases, and ensure the accuracy of critical test results that guide treatments and save lives. So the irony here is striking. Because without medical lab scientists, doctors would be left guessing. Yet, in public discourse, media, and even in healthcare celebrations, we remain in the shadows.

Well, as a friend usually says, "there will always be someone who cannot see your worth, but don't let that person be you". So maybe we should stop waiting for people to put a spotlight on us. Maybe it's time we switch on our own lights on and let it shine.

Shine your light!

©benjasco

02/06/2024

Just so you know your importance in the healthcare sector.

24/04/2024

Shout out to my newest followers! Excited to have you onboard! Erfan Erfan, Steve Bala Dawam, Queen Nk Roland, Bala Yusuf Ali, Maku Akwashiki, Zigwai Bonet, Djsmilez Jonathan, FA RU QU, Prince Larry Gold, Kenny Lyam

22/04/2024

Candida parapsilosis

22/04/2024

TYPES OF CULTURE MEDIA BASED ON OXYGEN REQUIREMENT.

Microorganisms have different requirements for growth depending on oxygen requirements.

1. Aerobic media
In this media, it is easy to cultivate microbes, on solid media, the growth occurs by keeping the culture in the incubator. It shows the growth; of non-fastidious microorganisms.

Examples of aerobic media are- liquid media, solid media

Peptone water- 1%peptone + 0.5% Nacl +100ml water.

Nutrient agar- nutrient broth +2% agar.

2. Anaerobic media
The media cultivates anaerobic bacteria at low oxygen, reducing oxidation-reduction potential. Anaerobic media contains extra nutrients like vitamin K, hemin, and oxygen that get reduced by a physical or chemical process. The addition of glucose (1%), thioglycollate(0.1%), ascorbic acid (0.1%), cysteine (0.05%), or iron fillings added to cause the medium to reduce. The medium is boiled in a water bath to force out dissolved oxygen and packed with sterile paraffin.

Examples of Anaerobic media:

RCM (Robertson cooked meat) isolation for Clostridium sp.

Thioglycolate broth– It has sodium glycolate that maintains low oxygen.

22/04/2024

HOW TO PREPARE CULTURE MEDIA?

-Weigh the amount of ingredients powder on weighing machine.

-Dissolve the ingredients in distilled water.

-Adjust PH of the medium if needed.

-Add agar and boiled it to dissolve.

-Pour the media into flask.

-Autoclave the media when ingredients fully dissolve.

-Sterilization is done in autoclave to prevent from contamination, at 121ºC for 15 min at 15lbs.

-After the autoclave place the media flask in laminar air flow.

-Sterilize the laminar air flow with 70% alcohol.

-A bit cools down the media and pours into sterile Petri-plates for solidification.

-Then sample is ready to spread(spreader) / streak

-(Inoculation loop) on the medium for identification or isolation of microbes.

-Sealed the Petri plates with paraffin, label them.

-Keep them inverted in incubator at 37ºC for 24hrs.

-Observe the result next day colonies formation is visible on the media.

22/04/2024

WHAT IS CULTURE MEDIA?

The media is a source of nutrients to support the growth of the micro-organisms in-vitro. The media helps in the growth and counting of microbial cells, selection of microorganisms, and survival of microorganisms. The culture medium can be liquid or gel.

COMMON INGREDIENTS OF CULTURE MEDIA

Peptone- source of carbon and nitrogen.

Beef extract- source of amino acid, vitamins, minerals.

Yeast extract- source of vitamin, carbon, nitrogen.

Distilled water

Agar- solidifying agent.