Medical Laboratory Science Forum

18/06/2025

What a worrisome discovery that is dangerous for the future existence of the males

Males have a heterozygous chromosomal pair XY and females with homozygous pair XX. To have a male child, during conception the zygote formed must have inherited the Y chromosome from the Father and the X from the mother (XY) while for a female child the zygote inherits X from both parents (XX)

The X & Y chromosomes are s*x chromosomes that determine if a pregnancy would result in a male or female child, disappearing of Y chromosomes is a sign that in the future it will be difficult to have male children

Should it be said we are moving towards the era of producing females who would go in multitude and beg to be married by one man while the feed and clothes themselves as prophesied in the Bible?

Should we be thinking towards invitro technologies that can keep the Y chromosomes devoid of any chromosomal defect for future use ?

Should we encourage our young guys to marry and start having male children now before th Y chromosome is gone completely?

18/06/2025

You received a blood sample from ED with a form that is well detailed, but the sample itself is not labeled.

You have rejected the sample as required, but the requested Doctor calls you and said to you that he had made a mistake by not labeling the sample, provided all the patient information which sharply match the information on the form.

The Doctor said to you that he wants to come up to the lab and label the sample appropriately, that the patient is really difficult to bleed and he needs the result urgently.

What will you do?

17/06/2025

Platelet Distribution Width (PDW) test

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1. Objective

The objective of this test was to measure the variability in platelet size (anisocytosis of platelets), which provided information about platelet activation and production dynamics.

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2. Principle

The test was based on the principle that platelet size variation can be assessed using automated hematology analyzers. A wider distribution indicates heterogeneous platelet populations, which may be seen in disorders involving platelet destruction or production.

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3. Materials

EDTA-anticoagulated blood sample

Automated hematology analyzer

Quality control reagents

Sample tube (purple top)

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4. Procedure

1. The EDTA blood sample was collected and gently mixed.

2. It was loaded into an automated hematology analyzer.

3. The analyzer assessed platelet size variation and reported the PDW as part of the platelet histogram.

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5. Result

Normal range: 9 – 17 fL (may vary slightly with machine and lab)

Increased PDW: Associated with platelet activation, myeloproliferative disorders, ITP, and inflammation

Decreased PDW: Rare, may suggest uniform but low platelet production

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6. Uses

Assessed platelet size variability in conditions like thrombocytopenia, ITP, DIC, and bone marrow disorders

Helped differentiate between hypoproliferative vs hyperdestructive thrombocytopenia

Provided insight into platelet activation status

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7. Conclusion

PDW was a useful hematological parameter for evaluating platelet morphology and distribution, especially in conjunction with platelet count and MPV (mean platelet volume).
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Medical Laboratory Science Forum

17/06/2025

Urine slide
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Medical Laboratory Science Forum

17/06/2025

Here is the website for Nigerian medical laboratory technician council examination https://eduportal.mlscn.gov.ng/Home?ReturnUrl=%2f&AspxAutoDetectCookieSupport=1
Or inbox me with your details if you can't check your's fans
Medical Laboratory Science Forum

16/06/2025

Congratulations to newly Nigerian Mlt as April 2025 national exams is out fans

16/06/2025

16/06/2025

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Medical Laboratory Science Forum

16/06/2025

16/06/2025

1. Dispense 100 microliters (0.1ml) of capillary blood collected at the correct time into a centrifuge tube containing 1ml of saponin-saline lyzing solution or water
2. Mix gently and leave for about 2minutes for all red cells to lyze (leave for 10 minutes if using water)
3. Centrifuge at slow to medium speed i.e RCF 300-500g for 5minutes.
4. Remove and discard the supernatant using pasture pipette.
5. Transfer all the sediment to a slide, add a small drop of methylene blue-saline, Azure B, or Field's stain A and cover with a cover slip.
6. Examine the entire preparation microscopically for Microfilariae using 10x objective.
7. Count the number of microfilariae in the entire preparation. Multiply the number by 10 to give an approximate number of microfilariae per ml of blood (mf/ml).

RESULTS:
Positive result: report microfilariae seen given the name of the microfilariae present e.g Loa loa, Wucheria bancrofti, Brugia malayi etc.

Negative result: Report No Microfilariae seen

16/06/2025

Malaria parasite P. vivax
Plasmodium vivax is a protozoal parasite and a human pathogen. This parasite is the most frequent and widely distributed cause of recurring malaria.

15/06/2025

urine sample??

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