24/09/2025
Semen Analysis
1. Objective
The objective of the semen analysis was to evaluate the quality and fertility potential of a male semen sample by examining its physical, chemical, and microscopic characteristics.
2. Principle
The principle was that semen quality could be determined by assessing physical parameters (volume, color, viscosity, pH), motility of s***matozoa, concentration (s***m count), morphology, and the presence of abnormal cells or contaminants. These parameters reflected the functional capacity of the male reproductive system.
3. Materials
• Sterile wide-mouth semen collection container
• Microscope (light microscope with phase-contrast if available)
• Glass slides and coverslips
• Counting chamber (e.g., Neubauer chamber)
• pH paper or pH meter
• Timer/stopwatch
• Personal protective equipment (gloves, lab coat, mask)
• Incubator (if needed for liquefaction observation)
4. Procedure
1. The semen sample was collected after 3–5 days of abstinence, by ma********on into a sterile container.
2. The sample was allowed to liquefy at room temperature for 30 minutes.
3. Physical examination was performed, noting volume, color, viscosity, and pH.
4. A drop of semen was placed on a slide, covered, and observed under the microscope for motility (progressive, non-progressive, immotile).
5. The s***m concentration was determined using a Neubauer chamber.
6. Smears were prepared and stained for assessing s***m morphology.
7. The presence of pus cells, red blood cells, or epithelial cells was also recorded.
5. Result
• Normal findings (WHO reference values):
o Volume: ≥1.5 mL
o pH: 7.2–8.0
o S***m count: ≥15 million/mL
o Motility: ≥40% motile
o Morphology: ≥4% normal forms
• Abnormal findings included low volume, low s***m concentration (oligos***mia), absent s***m (azoos***mia), poor motility (asthenozoos***mia), or abnormal morphology (teratozoos***mia).
6. Uses
• It was used for male infertility evaluation.
• It helped monitor the success of vasectomy
