24/08/2025
As I continue learning, it becomes evident that medicine is always evolving and sometimes practices differ depending on context and local resources.
Amniocentesis can be considered in patients with preterm labor without a determined cause. This is a standard approach in international guidelines but not usually done in our country.
Of course, the application depends on what’s available. 👍
Where nanopore sequencing isn’t accessible, sending amniotic fluid for culture and standard tests can still be very useful.
The rapid diagnosis of intraamniotic infection with nanopore sequencing - Classification of the study groups: amniotic fluid represents no intraamniotic inflammation (gray) and amniotic fluid represents intraamniotic inflammation (red). Definitions of the study groups: 1) no intraamniotic infection or intraamniotic inflammation: no microorganisms in the amniotic cavity in the absence of intraamniotic inflammation; 2) microbial invasion of the amniotic cavity without intraamniotic inflammation (MIAC): the presence of amniotic fluid microorganisms in the absence of intraamniotic inflammation; 3) sterile intraamniotic inflammation: intraamniotic inflammation in the absence of microorganisms; and 4) intraamniotic infection: intraamniotic inflammation with the presence of bacteria. Microorganisms were detected by either culture or 16S Sanger sequencing. Intraamniotic inflammation is defined by an amniotic fluid interleukin 6 concentration ≥2.6 ng/mL.
MIAC, microbial invasion of the amniotic cavity. https://ow.ly/yQ4G50WL11O
