04/08/2025
🔬💡 Why Use♦️ CLED Agar ♦️in Urine Microbiology❓
When it comes to isolating and identifying urinary pathogens, CLED (Cystine Lactose Electrolyte Deficient) agarremains a gold-standard medium in diagnostic microbiology.
Here’s why 👇
🧫 Top 3 Functional Advantages of CLED Agar:
1️⃣ Swarming Inhibition:
No more Proteus spp. taking over your plate!
🦠 CLED suppresses Proteus mirabilis swarming, allowing clear differentiation of individual colonies.
📚 According to Cheesbrough (2019), CLED's selective formula ensures better colony isolation compared to MacConkey in mixed cultures.
2️⃣ Lactose Fermentation Clarity:
🎨 Visual color indicators aid rapid interpretation:
✅ Yellow = Lactose fermenters (E. coli, Enterococcus)
✅ Blue/Green = Non-fermenters (Proteus, Pseudomonas)
📚 A study published in the African Journal of Clinical and Experimental Microbiology (2020) found CLED agar had a 94.6% detection rate for UTI pathogens, significantly aiding empirical treatment decisions.
3️⃣ Electrolyte Deficiency = Balanced Growth:
By limiting electrolytes, the medium prevents overgrowth of fast-growing organisms, enabling polymicrobial detectionwithout masking smaller colonies.
⚕️ Clinical Relevance:
Urinary tract infections (UTIs) are one of the most common bacterial infections worldwide, affecting over 150 million people annually (WHO, 2023).
🚨 Causative agents often include:
🔹 E. coli
🔹 Proteus spp. – notorious for urease activity and kidney stone formation
🔹 Staphylococcus aureus – may indicate hematogenous spread if found in urine
✅ In such cases, accurate colony identification is essential for guiding treatment, especially in polymicrobial samples.
🧪 BONUS – Aztreonam Disk Selectivity Insight:
🧑🏻🔬Here's a cool lab trick:
Use an Aztreonam disk on CLED agar.
S. aureus grows near the disk due to its intrinsic resistance
Gram-negative rods like Proteus mirabilis are inhibited
📸 This creates a selective zone, helping visually distinguish Gram-positive from Gram-negative organisms — smart, simple, and effective.
💬 Your Turn:
What media do you trust most for urine cultures? Any lab tips or insights from your bench work? Let’s share and learn 👇
゚viralシ