07/10/2025
CBC (Complete Blood Count) Test
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1. Objective
The objective of the test was to evaluate the overall health of the patient by measuring the levels of different blood cells including red blood cells, white blood cells, hemoglobin, hematocrit, and platelets.
2. Principle
The test was based on the principle of automated hematology analysis. Blood cells were counted and classified by electrical impedance and/or flow cytometry, while hemoglobin concentration was measured photometrically.
3. Materials
Patient’s venous blood sample (collected in an EDTA tube)
Sterile syringe and vacutainer
Automated hematology analyzer (CBC machine)
Microscope (for peripheral smear examination)
Glass slides, stains (Wright’s or Giemsa stain)
Personal protective equipment (PPE)
4. Procedure (Microscopic and Automated)
A venous blood sample was collected in an EDTA tube.
The sample was loaded into an automated hematology analyzer.
The analyzer counted RBCs, WBCs, and platelets using impedance or laser flow methods.
Hemoglobin concentration was measured spectrophotometrically.
Hematocrit and red cell indices (MCV, MCH, MCHC) were calculated.
A peripheral blood smear was prepared, stained, and examined microscopically for confirmation of abnormal findings.
5. Result
The results were displayed as numerical values and histograms for RBCs, WBCs, platelets, hemoglobin, and hematocrit.
Microscopy confirmed morphology of cells in case of abnormalities.
Normal values were compared with standard reference ranges (which varied with age and s*x).
6. Uses
It was used to diagnose anemia, infections, and many blood disorders.
It was used to monitor treatment response in patients with chronic illnesses.
It was used as a routine check-up for general health screening.
7. Consultation
The results were interpreted by a physician. Abnormal results were correlated with clinical history, symptoms, and other investigations. The patient was advised on further tests or treatment if neces