19/01/2025
𝘾𝘼𝙈𝙋 𝙏𝙚𝙨𝙩📌
▪️It is used for the identification of 𝙎𝙩𝙧𝙚𝙥𝙩𝙤𝙘𝙤𝙘𝙘𝙪𝙨 𝙖𝙜𝙖𝙡𝙖𝙘𝙩𝙞𝙖𝙚.
• It is a Group 𝐁 𝐛𝐞𝐭𝐚-𝐡𝐞𝐦𝐨𝐥𝐲𝐭𝐢𝐜 streptococci.
• It is the only beta-hemolytic Streptococcus which secrete a protein called 𝐂𝐀𝐌𝐏 𝐟𝐚𝐜𝐭𝐨𝐫 𝐨𝐫 "𝐩𝐫𝐨𝐭𝐞𝐢𝐧 𝐁".
• It is one of the causative agents of 𝐦𝐚𝐬𝐭𝐢𝐭𝐢𝐬 𝐢𝐧 𝐜𝐨𝐰𝐬.
▪️ 𝘾𝘼𝙈𝙋 is an acronym for the authors of this test (𝐂𝐡𝐫𝐢𝐬𝐭𝐢𝐞, 𝐀𝐭𝐤𝐢𝐧𝐬𝐨𝐧, 𝐌𝐮𝐧𝐜𝐡, 𝐚𝐧𝐝 𝐏𝐞𝐭𝐞𝐫𝐬𝐨𝐧) which was identified in 1944.
𝗣𝗿𝗶𝗻𝗰𝗶𝗽𝗹𝗲📌
• Streptococcus agalactiae produces a diffusible, thermostable, extracellular protein known as CAMP factor.
▪️CAMP factor acts synergistically with the beta lysin produced by Staphylococcus aureus to produce a zone of enhanced lysis of sheep or bovine erythrocytes.
• The group B streptococci are streaked 𝐩𝐞𝐫𝐩𝐞𝐧𝐝𝐢𝐜𝐮𝐥𝐚𝐫 𝐭𝐨 𝐚 𝐬𝐭𝐫𝐞𝐚𝐤 𝐨𝐟 𝐒. 𝐚𝐮𝐫𝐞𝐮𝐬 on sheep blood agar.
𝗥𝗲𝘀𝘂𝗹𝘁𝘀📌
• A 𝐩𝐨𝐬𝐢𝐭𝐢𝐯𝐞 𝐫𝐞𝐚𝐜𝐭𝐢𝐨𝐧 appears as an 𝐞𝐧𝐡𝐚𝐧𝐜𝐞𝐝 𝐡𝐞𝐦𝐨𝐥𝐲𝐬𝐢𝐬 is indicated by an arrow head-shaped zone of beta-hemolysis at the junction of the two organisms.
• Negative: No 𝐞𝐧𝐡𝐚𝐧𝐜𝐞𝐦𝐞𝐧𝐭 𝐨𝐟 𝐡𝐞𝐦𝐨𝐥𝐲𝐬𝐢𝐬.
𝙐𝙨𝙚𝙨 𝙤𝙛 𝘾𝘼𝙈𝙋 𝙩𝙚𝙨𝙩📌
▪️ It can be used to determine the ability of an organism to produce the CAMP factor.
▪️ It is used to differentiate Streptococcus agalactiae from other species of beta-hemolytic Streptococcus.
▪️It is used to identify Listeria monocytogenes which also produces a positive CAMP reaction.