The Medical Lab Sialkot

14/07/2025

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27/06/2025

Reticulocyte Count

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1. Objective

The objective of this test was to measure the percentage of reticulocytes (immature red blood cells) in the blood, providing information about bone marrow activity and RBC production.

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2. Principle

Reticulocytes were identified using a supravital stain (e.g., new methylene blue) that highlighted residual RNA. The stained cells were then counted under a microscope and expressed as a percentage of total RBCs.

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3. Materials

EDTA blood sample

New methylene blue stain

Microscope slides and cover slips

Microscope

Mixing sticks or pipette

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4. Procedure

1. A few drops of EDTA blood were mixed with an equal amount of new methylene blue stain.

2. The mixture was incubated at room temperature for 10–15 minutes.

3. Thin blood smears were prepared on slides from the stained mixture.

4. The smears were dried and examined under the microscope using oil immersion.

5. Reticulocytes were counted in a total of 1000 RBCs, and the percentage was calculated.

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5. Result

Normal Range:

Adults: 0.5%–2.5%

Newborns: 2%–6%

Increased Reticulocyte Count:

Hemolytic anemia

Blood loss

Recovery from anemia

Decreased Reticulocyte Count:

Aplastic anemia

Bone marrow failure

Iron/B12/folate deficiency

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6. Uses

Assessed bone marrow function

Helped evaluate response to anemia treatment

Distinguished between hypoproliferative and hyperproliferative anemias

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7. Conclusion

The test effectively indicated bone marrow activity and was critical in the diagnosis and monitoring of anemia and bone marrow disorders.

05/06/2025

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05/06/2025

12/05/2025

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05/05/2025

01/05/2025

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30/03/2025

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08/03/2025

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