27/06/2025
Reticulocyte Count
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1. Objective
The objective of this test was to measure the percentage of reticulocytes (immature red blood cells) in the blood, providing information about bone marrow activity and RBC production.
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2. Principle
Reticulocytes were identified using a supravital stain (e.g., new methylene blue) that highlighted residual RNA. The stained cells were then counted under a microscope and expressed as a percentage of total RBCs.
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3. Materials
EDTA blood sample
New methylene blue stain
Microscope slides and cover slips
Microscope
Mixing sticks or pipette
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4. Procedure
1. A few drops of EDTA blood were mixed with an equal amount of new methylene blue stain.
2. The mixture was incubated at room temperature for 10–15 minutes.
3. Thin blood smears were prepared on slides from the stained mixture.
4. The smears were dried and examined under the microscope using oil immersion.
5. Reticulocytes were counted in a total of 1000 RBCs, and the percentage was calculated.
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5. Result
Normal Range:
Adults: 0.5%–2.5%
Newborns: 2%–6%
Increased Reticulocyte Count:
Hemolytic anemia
Blood loss
Recovery from anemia
Decreased Reticulocyte Count:
Aplastic anemia
Bone marrow failure
Iron/B12/folate deficiency
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6. Uses
Assessed bone marrow function
Helped evaluate response to anemia treatment
Distinguished between hypoproliferative and hyperproliferative anemias
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7. Conclusion
The test effectively indicated bone marrow activity and was critical in the diagnosis and monitoring of anemia and bone marrow disorders.
