Green Culture Labs co., Ltd

30/12/2025

Life Time Cannabis Use Shows Larger Brain Volumes in Receptor Regions Rich in Cannabinoid

History of Cannabis Use and Cognitive Function in Older Adults: findings from the UK BioBank

A large observational analysis using data from UK Biobank examined cannabis use and brain health in more than 500,000 adults aged 40 to 70.

The findings challenge common assumptions about cannabis effects later in life.

✔️ Brain Structure

• Lifetime cannabis users showed larger volumes in brain regions rich in cannabinoid receptors

• Regions included the caudate, putamen, hippocampus, and amygdala

• Even people who used cannabis only during adolescence showed larger volumes than non users

Note: Adolescent cannabis use is not recommended (without medical professionals), with some studies showing early exposure linked to adverse cognitive and mental health outcomes.

✔️ Cognitive Performance

• Greater lifetime use associated with better learning ability

• Faster processing speed observed

• Improved short term memory performance noted

✔️ S*x Differences

• Brain and cognitive associations differed between men and women

• Highlights the need to consider biological s*x in cannabis research

✔️ Proposed Biological Mechanisms

• Modulation of the endocannabinoid system may reduce neuroinflammation

• Improved immune regulation may support neural resilience

• Possible slowing of neurodegenerative processes

✔️ Key Limitations

• Observational data show association not causation

• Genetics, dose, frequency, product composition, and method of use not fully captured

• Lifestyle and socioeconomic factors may influence outcomes

• Self selection bias likely among long term users

✔️ Genetic Diversity and Individual Response

• Genetic variation strongly influences cannabinoid receptor density and function

• Differences in cannabinoid metabolism affect efficacy and side effects

• Baseline endocannabinoid system activity is highly individualized

✔️ Implications for the Future

• Personalized approaches are likely essential for safe and effective cannabis based therapies

• Genetic and biological profiling may help guide compound selection and dosing

• Individualized treatment could maximize benefit while minimizing risk

✔️ Context Matters

• Findings differ from studies showing harm with adolescent cannabis exposure

• Effects appear to depend on age of use, biology, and broader life context

Conclusion

This large population study suggests that in mid to later adulthood, cannabis use is associated with preserved brain volume and certain cognitive advantages.

The relationship is complex, highly individualized, and shaped by genetics, s*x, timing of exposure, and product characteristics (cultivars unique profiles).

Moving toward personalized cannabis compounds (full spectrum - specific cultivars specifically for brain health) medicine may unlock benefits for brain while reducing risk, but requires careful scientific validation and responsible clinical application.

With over 500,000 participants, the observed correlation between lifetime cannabis use and larger brain volumes in regions rich in cannabinoid receptors is particularly intriguing, given existing evidence that multiple cannabis compounds may exert neuroprotective effects.

These potential protective mechanisms include modulation of the endocannabinoid system, reduction of neuroinflammation, antioxidant activity, regulation of glutamate excitotoxicity, support of mitochondrial function, promotion of synaptic plasticity, and indirect benefits through improved stress regulation and sleep, suggesting a biologically plausible basis for the associations observed.

Cultivating Cannabis Cultivars Profiles Best for Brain Health

December 2025
Link 🔗 to full Study in comments based on UK BioBank

29/12/2025

New Key Active Odor Discovery in Cannabis Sativa L. For First Time in Flowers.

Hidden Aroma Compounds Explain Why Cannabis Sativa L. Smells the Way It Does: Skunk / Gassy Smells

First Quantitation of Key Odor-Active Thiols in Flowers of Different H**p Cultivars Using HPLC-MS/MS

✔️ Study Overview

• Researchers developed a highly sensitive analytical method to measure sulfur containing aroma compounds in h**p flowers

• These compounds occur at extremely low levels but have a major impact on smell and perceived quality

✔️ Why Sulfur Compounds Matter

• Sulfur containing molecules known as thiols have very strong odors even at trace concentrations

• They contribute to skunky gassy and pungent notes often noticed in h**p and cannabis

• Traditional terpene testing usually misses these compounds

✔️ Analytical Innovation

• The team used a chemical derivatization step to stabilize fragile thiols so they could be measured reliably

• Stable isotope labeled internal standards were added to ensure accurate quantification

• A gentle extraction technique was used to preserve volatile aroma compounds

• Results were confirmed using an independent gas chromatography based method

✔️ Key Findings

• Detection limits reached the low nanogram per kilogram range demonstrating exceptional sensitivity

• Several sulfur aroma compounds were quantified in h**p for the first time

✔️ Quantified in h**p for the first time (validated quantitative data)

• 3-methylbut-2-ene-1-thiol
• Other low-level sulfur thiols included in the analytical panel

Reported for the first time ever in h**p flowers
• 4-methyl-4-sulfanylpentan-2-one

✔️ Scientific Significance

• Confirms that aroma is not determined by abundance alone but by compounds with very low odor thresholds

• Shows that sulfur chemistry plays a critical role alongside terpenes in h**p aroma

• Moves h**p aroma science from descriptive smelling to measurable chemistry

✔️ Practical Applications

• Enables objective comparison of aroma profiles between cultivars

• Supports breeding programs targeting specific aroma traits: More or Less of that Gas / Skunk

• Provides a new quality control tool for post harvest handling storage and processing

• Helps explain why products with similar terpene profiles can smell very different

✔️ Big Picture Takeaway

• Advanced analytical methods are revealing hidden drivers of h**p aroma

• Sulfur containing compounds deserve attention alongside terpenes in research and quality control

• This work sets a new benchmark for precision aroma analysis in botanicals

Taste - Smell - & Structure in search for your satisfying Cannabis Sativa L.

December 26, 2025
Link 🔗 to full study in comments

28/12/2025

Cannabinoids and Dental Health: CBD - CBG

Medical Research examines how non-psychoactive compounds from cannabis may help prevent tooth decay.

Key Findings

✔️ How They Work

• Cannabinoid receptors are present throughout oral tissues and salivary glands

• These compounds target the main bacteria responsible for cavities: Streptococcus mutans and Lactobacillus

• They work through antimicrobial, antibiofilm, anti-inflammatory, and antioxidant properties

✔️ Cannabidiol (CBD) Effects

• Inhibits acid production by cavity-causing bacteria

• Reduces biofilm formation by over 90%

• Shows effectiveness against drug-resistant bacteria

• Does not lead to bacterial resistance with regular use

✔️ Cannabigerol (CBG) Effects

• Achieved over 98% bacterial reduction within 8 hours at specific concentrations

• Demonstrates both bacteria-stopping and bacteria-killing properties

• Prevents biofilm formation on teeth

✔️ Clinical Performance

• Cannabinoid mouthwashes perform similarly to chlorhexidine, a standard antimicrobial

• May outperform some commercial oral hygiene products in reducing dental plaque bacteria

• Unlike traditional antimicrobials, they appear less likely to cause bacterial resistance

✔️ Other Dental Applications Being Studied

• Tooth pain management
• Periodontal disease treatment
• Gum infections
• Burning mouth syndrome
• Dental anxiety reduction

Current Status

These products remain in developmental stages. The FDA has not yet established a regulatory framework and considers them unsafe without medical supervision.

Additional research is needed to fully understand their mechanisms, long-term safety, and potential side effects before clinical use.

Schedule 3 for Cannabis Legislation may have positive implications for similar Dental and Medical Care with cannabinoids.

Cannabis - Kratom - and Psychedelic Mushrooms containing various and valuable active compounds.

Proper (Plant) Protocols and Precise Dosing

Tailored to each individual’s unique needs, proving that plants and fungi offer a wide range of medical applications with equal potency and efficacy, safer profiles, and fewer adverse effects compared to conventional treatments.​​​​​​​​​​​​​​​​

December 2025
Source: Cihangir & Acarturk, UKR Journal of Medicine and Medical Research, 2025​​​​​​
Link 🔗 to full read in Comments

26/12/2025

A Potent Plant with Plethora of Potentials for People and Planet

A Flower by Any Other Name:
The Case for Cannabis Right to Farm Protection

✔️ Genetic Similarity

• H**p and intoxicating cannabis show less genetic difference than varieties of maple trees

• Both are Cannabis sativa, differentiated only by a 0.3% THC threshold

• THC itself is odorless; both plants produce similar aromatic compounds

Current Legal Disparities

✔️ H**p Benefits

• Access to USDA loans and technical support

• Small Business Administration assistance

• Eligibility for organic certification

• Federal bankruptcy protection

✔️ Cannabis Exclusions

• No access to federal agricultural programs

• Limited banking and lending options

• Subject to restrictive zoning that increases costs
Environmental Impact of Current Policy

✔️ Indoor Cultivation Problems

• Indoor cannabis operations account for 1.3% of Colorado greenhouse gas emissions

• Comparable to coal mining emissions in the same state

• Creates concentrated pollution in urban areas

• Increases worker exposure to chemicals and mold

✔️ Geographic Concentration

• Restrictive zoning forces facilities into industrial zones

• Operations concentrated in low income minority neighborhoods

• Artificially inflated land prices in permitted zones

The Right to Farm Solution

✔️ What It Provides

• Rebuttable presumption against nuisance lawsuits for agricultural activities

• Protection for established farms from later arriving neighbors

• Currently applies to h**p and other crops in all 50 states

• Would require objective standards for complaints while maintaining oversight

✔️ Implementation Requirements

• Define cannabis cultivation as agricultural rather than industrial activity

• Establish good agricultural practices standards

• Create objective nuisance measurements like odor dilution ratios

• Maintain public health and safety enforcement for actual violations

Equity and Market Implications

✔️ Current Barriers

• High compliance costs favor large operators over small farmers

• Minority entrepreneurs face disproportionate obstacles

• Indoor facility requirements increase capital needs substantially

✔️ Potential Benefits

• Lower barriers to entry for disadvantaged communities

• Reduced environmental concentration in minority neighborhoods

• Greater opportunities for outdoor cultivation with lower costs

✔️ Market Stability

• High barriers in legal market drive continued illicit activity

• Estimated two thirds of California cannabis purchases occur in unregulated market

• Clear agricultural standards could improve compliance rates

✔️ Key Takeaway

The artificial legal distinction between h**p and intoxicating cannabis creates unnecessary regulatory complexity that undermines both agricultural preservation and public safety goals.

Seeds of Change: Planting the Right to Farm for All Cannabis Sativa L.

December 2025
Full Legal Documents (Call To Action) in Comments

24/12/2025

Happy Holidays everyone!

To new beginnings and to old friends, new friends, and all the wonderful people in our lives who truly matter.

My prayers this season continues to be: for world peace, that the hungry be fed, the lost be found, the sad find joy, and those being harmed receive the help they need.

No matter what country, culture, color, religion, or creed, I believe in the universal power of prayer.

I truly believe and hold onto hope that when we speak these words repeatedly and shared often enough, they will be heard and answered. Thank you

Respectfully,

Thank you Truong M Nguyen

Green Culture Labs co., Ltd

23/12/2025

22/12/2025

Cannabis Sativa L. Terpene in Cow Milk Terpene Profiles: Impact of H**p and Savory Supplementation

The research focused on the transfer efficiency and kinetics of terpenes which are secondary metabolites responsible for the distinct sensory and bioactive properties of aromatic plants.

Leaves and stems of non-flowering plants contain measurable terpene levels, though concentrations are lower than in flowers.

✔️ Study Design and Methodology
Research Parameters

• Six Simmental dairy cows were used in a 3 x 3 Latin square design.

• Dietary treatments included a control Total Mixed Ration TMR and TMR supplemented with fresh h**p or savory leaves.

• Supplementation followed a step up regimen from 0.2 to 0.8 kg per day of dry matter to allow for animal adaptation.

• Terpenes were analyzed using headspace solid phase microextraction HS SPME and gas chromatography mass spectrometry GC MS.

✔️ Key Research Findings
Terpene Identification and Concentration

• A total of 24 terpenes were identified across the feed and milk samples.

• H**p leaves exhibited the highest total terpene content at 85,962 micrograms per kg.

• Savory leaves contained 11,313 micrograms per kg while the control diet contained 228 micrograms per kg.

• Milk from h**p fed cows showed the highest absolute terpene concentration at 388.8 micrograms per kg.

✔️ Transfer Dynamics and Efficiency

• The relative carryover rate was highest in the control group at 15.4 percent compared to 8.7 percent for h**p and 6.2 percent for savory.

• An inverse relationship was observed where higher concentrations in feed often resulted in lower transfer efficiency to the milk.

• Major compounds
successfully transferred included alpha farnesene, beta caryophyllene, and p cymene.

• High transfer rates for specific compounds like alpha citral suggest potential endogenous transformation by rumen microbes.

✔️ Kinetics and Persistence Elimination Rates

• Terpene levels in milk rose rapidly during supplementation and declined sharply once the herbs were removed.

• The half life of p cymene was calculated at approximately 48 hours.

• Sesquiterpenes such as alpha farnesene and beta caryophyllene showed shorter half lives between 16 and 18 hours.

• The rapid clearance indicates that terpenes do not have a memory effect in the animal body and require continuous ingestion for milk enrichment.

Practical Implications for the Dairy Industry

✔️ Product Value and Traceability

• Using terpene rich plants can naturally enhance the sensory profile and nutritional value of dairy products.

• Terpenes serve as effective biomarkers for verifying the dietary history and origin of milk.

• H**p supplementation specifically offers a high yield of sesquiterpenes which are associated with unique aromatic qualities.

• The findings support the development of sustainable dairy systems focused on differentiated high quality milk products.

Previously Studies RnD conducted on Cannabis sativa L. feed supplementation in cattle, sheep, goats, pigs, poultry, fish, and horses.

Common Benefits Across Species

1. Nutritional enhancement: Rich in protein (25-35%), essential amino acids, and beneficial omega-3 and omega-6 fatty acids

2. Antioxidant properties: Increased antioxidant status and reduced oxidative stress

3. Anti-inflammatory effects: Improved fatty acid profiles supporting reduced inflammation

4. Stress reduction: Calming effects observed in multiple species

5. Improved product quality: Enhanced omega-3 content in meat, milk, and eggs

6. Environmental benefits: Reduced methane emissions in ruminants and pigs

Share your thoughts and experiences of Cannabis Sativa L. as Animal Feed.

Available Online:
December 18, 2025
Link 🔗 to Source in Comments