12/15/2025
WHAT ARE NANOFLOWERS?
Nanoflowers are next-generation flower-shaped hybrid nanoparticles engineered to:
Increase mitochondrial ATP production
Reduce oxidative stress (ROS)
Improve cellular resilience
Deliver bioactive peptides + antioxidants directly to mitochondria
Their unique petal-like nanosheet architecture allows ultra-high catalytic power.
HOW THEY ARE MADE
Self-Assembled Hybrid Nanostructures
Metal Ions + Peptides/Proteins + Phosphate → Nanoflower Growth
Metal ions (Cu²⁺, Zn²⁺, Ce³⁺, Ca²⁺)
Bioelectric-responsive peptides or targeting proteins
Phosphate buffer
Room-temperature self-assembly forms flower-like nanosheets
Surface functionalization with TPP⁺ or mitochondrial-targeting peptides (MTPs) directs them into mitochondria
HOW THEY WORK
BIOELECTRICALLY ENHANCED MITOCHONDRIAL SUPPORT
Nanozyme Catalysis (Artificial Enzyme Activity)
SOD-like → clears superoxide
Catalase-like → breaks down H₂O₂
Peroxidase-like → removes peroxides
➤ Protects mitochondria + preserves membrane potential
Faster Electron Transport (ETC Improvement)
Nanoflowers optimize:
NAD⁺/NADH balance
Complex I & Complex IV efficiency
Cytochrome c oxidase activity
➤ More ATP created per oxygen molecule
Targeted Delivery Inside Mitochondria
Surface functionalization with:
TPP⁺ mitochondrial targeting
Bioelectric-responsive peptides
Klotho-activating peptides
➤ Ensures precise intracellular delivery + slow release.
Stimulates Mitochondrial Biogenesis
Activation of:
PGC-1α
NRF1 / NRF2
TFAM
➤ Increases mitochondrial number & durability
BIOELECTRIC SYNERGY
When combined with Lionheart bioelectric therapies (BodStim™, BrainBand™, TestiStim™, ErectiStim™, HairCell™):
Ion channels open
Calcium oscillations improve
Intracellular uptake increases
Peptide + nano-delivery efficiency rises
Bioelectric stimulation is intended to amplifiy nanoflower performance.
Designed for:
SYSTEM-WIDE HEALTHSPAN IMPROVEMENT
Mitochondrial Biogenesis
Activation of PGC-1α, NRF1/2, TFAM, leading to new mitochondrial formation.
Reduction of Cellular Senescence
Lower oxidative stress reduces p16/p21 pathways and senescence-associated β-gal.
Stabilization of Biological Age
Combined mitochondrial repair + Klotho enhancement reduces multi-system aging markers.
What This Combination Platform Technology Is Intended to Achieve:
Increase whole body Klotho levels, a master longevity healthspan protein
Boost ATP production
Restore mitochondrial membrane potential
Reduce ROS & inflammation
Enhance autophagy & cellular repair
Improve stem cell function
Combined Mechanism of Action
Bioelectric stimulation increases nanoflower entry through voltage-gated Ca²⁺ channel modulation.
Nanoflowers act as nanozymes, reducing ROS and stabilizing mitochondrial membrane potential.
Functionalized nanoflowers deliver Klotho-modulating agents locally and intracellularly.
Bioelectric signals enhance mitochondrial transport, improving targeting efficiency.
Bioelectric signals increase Klotho transcription via frequency-specific epigenetic modulation.
Result: Improved mitochondrial function, reduced inflammation, enhanced autophagy, delayed senescence, and extended healthspan.
The newly filed patent protects a transformative therapeutic system that combines nanoflower-format nanoparticles, bioelectric signaling sequences, and Klotho-modulating biologics to target the cellular and mitochondrial drivers of aging.
