01/17/2024
Phases of Tendon Healing 🦶
https://link.springer.com/chapter/10.1007/978-3-662-54184-5_4?fbclid=IwAR29o-3gz5NQoG9mUK_YrN7zFf_jPJsjRbcer8XAj25jbBuscyp9jMaAN1w
🦶 The biologic cascade that modulates tendon healing is divided in overlapping phases, each governed by different cell types (https://pubmed.ncbi.nlm.nih.gov/15634833/, https://pubmed.ncbi.nlm.nih.gov/24057354/.
1⃣ The inflammatory phase (a) is initiated by the traumatic event surrounding tendon injury, precipitating the hematoma and the agglomeration of platelets, releasing a slew of chemotaxic molecules, cytokines, and growth factors.
👉 The phagocytic cells, monocytes, neutrophils, and microphages, migrate to the site of injury via dilated vessels and begin the process of breaking down the blood clot and extracellular matrix. The process of angiogenesis is introduced by macrophages where a new network of vasculature matures in the healing tissue (https://pubmed.ncbi.nlm.nih.gov/1383229/).
👉 The ECM is notably stabilized by the increasing amount of collagen type III, which are not yet aligned in parallel. The inflammatory stage lasts between 3 to 7 days following tendon injury. The amount of collagen will steadily increase during the first 5 days where the tendon callus will reach its largest size (https://pubmed.ncbi.nlm.nih.gov/21822105/)
2⃣ The proliferative phase (b) follows with an increasing amount of intrinsic fibroblast in the ECM. These cells emerge from the endotenon and epitenon and play a role in resorbing and producing new collagen (https://pubmed.ncbi.nlm.nih.gov/24057354/). At this stage, the immature healing ECM is still stabilized by a soaring amount of type III collagen, and this phase lasts for about 6 weeks.
3⃣ During the remodeling phase (b), the biomechanical strength of the tendon is at its greatest. The collagen is reorganized in a longitudinal, parallel fashion, while the collagen type III is replaced by collagen type I. The ECM will continue to mature for the next year with a rising amount of longitu-dinal and cross-linked collagen, while the callus volume of the tendon will decrease and the tendon biomechanical strength will continue to improve (https://pubmed.ncbi.nlm.nih.gov/21368094/).
📌 The healed tendon is biomechanically weaker than the uninjured tendon, with less cross-linking and smaller diameter collagen, and is more susceptible to reinjury. (https://pubmed.ncbi.nlm.nih.gov/10810761/). In a mice model, Dyment et al. (https://pubmed.ncbi.nlm.nih.gov/21698662/) demonstrated that the healed tendon regains 63% of its original strength after an 8-week time.
Illustration: https://link.springer.com/chapter/10.1007/978-3-662-54184-5_4?fbclid=IwAR29o-3gz5NQoG9mUK_YrN7zFf_jPJsjRbcer8XAj25jbBuscyp9jMaAN1w