01/08/2026
How does a UV-Vis Spectrophotometer work?
UV-Vis spectrophotometry is a powerful analytical technique used in various scientific fields to measure light absorbance across the electromagnetic spectrum’s ultraviolet (UV) and visible (Vis) ranges.
By measuring the intensity of light passing through a sample solution and comparing it to the intensity of the incident light, a UV-Vis spectrophotometer provides valuable information about the properties of materials and their interactions with light.
When incident light hits an object, it can be absorbed, reflected, or transmitted.
The spectrophotometer measures the intensity of light absorbed across the UV and Vis ranges.
Light transmitted through the sample is measured and compared to a reference measurement of the incident light source.
By applying the Beer-Lambert Law, which states that the amount of light absorbed is directly proportional to the concentration of the sample and the path length, the spectrophotometer can determine the concentration of specific analytes in the sample.